脑牵拉对兔脑神经元线粒体膜电位变化的影响

目的探讨脑牵拉压(BRP)的测量方法,及脑牵拉引起兔脑神经元线粒体膜电位变化的规律和机制。方法选用新西兰大白兔30只,平均随机分为30、40、50 g组,自制脑牵拉压测量装置并定标。流式细胞术检测各组脑牵拉压区神经细胞线粒体膜电位的变化。结果 30 g的 BRP牵拉30 min细胞线粒体膜电位为(97．14±2．27)％;40 g的BRP牵拉30 min引起细胞线粒体膜电位为(84．59±3．73)％,与30 g组相比明显降低,差异有统计学意义(P<0．01);50g的BRP牵拉15 min引起细胞线粒体膜电位为(45．28±3．51)％,与30 g组、40 g组相比差异具有统计学意义 (P<0．01)。结论该装置可精确测量脑牵拉压大小。脑牵拉可引起神经元细胞线粒体膜电位降低。实验性脑牵拉时,最好将BRP控制在40 g以下,以避免脑牵拉伤的发生。

Experimental study on the changes of cell mitochondrial membrane potential induced by brain retraction

Objective To investigate the method of measurement of brain retraction pressure (BRP) and observe the change of mitochondrial membrane potential (MMP) induced by brain retraction. Methods Thirty rabbits were randomly divided into 30 g (the brain retracted by 30 g pressure), 40 g (by 40 g pressure), 50 g (by 50 g pressure) groups. BRP was determined by self-made BRP instrument. The change of MMP was measured by flow cytometry. Results There was no change in MMP after retraction for 30 min in 30 g group. The slight decrease of MMP after retraction for 30 was observed in 40 g group. There was a significant decrease of MMP after retraction for 15 min in 50 g group. Conclusion This instrument can be used as a tool for measuring BRP. The brain retraction can induce the decrease of MMP. The BRP during the expezimental brain surgery should be less than 40 g to avoid brain injuries induced by the retraction.