羊水游离RNA中神经发育关键基因分析

目的分析孕中期羊水游离RNA(AfcfRNA)转录组,筛选神经发育共表达关键基因。方法利用基因共表达网络分析,建立AfcfRNA转录组的基因共表达网络模块。筛选各共表达模块中的神经特异性基因,建立神经系统特异性共表达模块。利用基因间相互作用筛选神经组织特异性共表达模块中的关键基因。结果通过加权基因共表达网络分析共建立27个以颜色命名的共表达模块,在Human Protein Atlas数据库中筛选到832个神经组织特异性基因。在蓝色、棕色、蓝绿色以及黄色模块中富集到前脑发育、神经突触组装和功能、神经递质释放过程、轴突发生以及学习和记忆过程相关的GO术语。通过基因间相互作用以及基因在孕中期的平均表达量分析,共发现蓝色模块(SLC18A3、TACR3、SYT2)、棕色模块(SSTR5、STX1A、SNAP25、GHSR、SSTR4、GABBR2)、蓝绿色模块(DRD2、SLC32A1、GNG3、OPN4、PENK)以及黄色模块(RAB3A、HCRT、GRM5)中的17个关键基因。结论该研究获得了神经系统发育密切相关的并且具有共表达关系的关键基因,可作为潜在的产前诊断中监测神经系统发育的标志物。

Analysis of hub genes of neurodevelopment in amniotic fluid cell free RNA

Objective To analyze the transcriptome of amniotic fluid cell free RNA(AfcfRNA)in the second trimester of pregnancy,and to screen the hub genes for co-expression in neurodevelopment.Methods The gene co-expression network module of AfcfRNA transcriptome was established by gene co-expression network analysis.The neural specific genes of the co-expression modules were screened,and the specific co-expression module of the nervous system was established.The hub genes in the neural tissue-specific co-expression module were screened by gene interactions.Results A total of 27 co-expression modules named by colors were established through weighted gene co-expression network analysis,and 832 neural tissue-specific genes were screened in the Human Protein Atlas database.GO terms related to forebrain development,neurosynaptic assembly and function,neurotransmitter release process,axogenesis,learning and memory process were enriched in blue,brown,blue-green and yellow modules.Through the analysis of gene interactions and the average expression of genes in the second trimester,a total of 17 hub genes were found in the blue module(SLC18A3,TACR3 and SYT2),the brown module(SSTR5,STX1A,SNAP25,GHSR,SSTR4 and GABBR2),the blue-green module(DRD2,SLC32A1,GNG3,OPN4 and PENK)and the yellow module(RAB3A,HCRT and GRM5).Conclusion This study has obtained the hub genes closely related to the development of nervous system and co-expression relationship,which can be used as a potential marker for monitoring nervous system development in prenatal diagnosis.