AHSG与CEA联合检测对膀胱癌的诊断价值

目的探讨胎球蛋白A(AHSG)与癌胚抗原(CEA)联合检测在膀胱癌诊断中的临床应用价值。方法选择2017年9月至2019年9月该院收治的30例膀胱癌患者作为膀胱癌组,另选择同期30例体检健康者作为健康组,采用酶联免疫吸附试验(ELISA)检测并比较2组血清AHSG及CEA水平。利用starBase和Oncomine数据库分析膀胱癌组织中的AHSG mRNA表达水平,利用Human Protein Atlas(HPA)数据库验证膀胱癌组织中的AHSG蛋白表达水平;利用Kaplan-Meier Plotter(KM Plotter)数据库分析AHSG预测膀胱癌患者预后的价值,采用受试者工作特征(ROC)曲线分析AHSG、CEA检测对膀胱癌的诊断价值。结果starBase和Oncomine数据库分析发现,膀胱癌组织中AHSG mRNA表达水平明显升高,且与患者的肿瘤分级、远处转移和淋巴结转移等密切相关(P<0.05)。HPA数据库验证了膀胱癌组织中AHSG蛋白表达水平较正常膀胱组织升高。ELISA结果显示,膀胱癌组患者血清AHSG、CEA水平较健康组明显升高(P<0.05)。KM Plotter数据库分析发现,AHSG高表达的膀胱癌患者较AHSG低表达患者具有更高的无复发生存概率。ROC曲线显示,AHSG与CEA联合检测诊断膀胱癌的曲线下面积为0.952,灵敏度为90.0%,特异度为93.3%,较AHSG、CEA单独检测对膀胱癌的诊断价值更高。结论AHSG在膀胱癌中的表达明显升高,是膀胱癌诊断和预后预测的客观评价指标,其与CEA是膀胱癌诊断和预后分析中临床应用价值更高的、潜在的血清学标志物。

Diagnostic value of combined detection of AHSG and CEA for bladder cancer

Objective To investigate the clinical application value of combined detection of Alpha-2-HS-glycoprotein(AHSG)and carcinoembryonic antigen(CEA)in the diagnosis of bladder cancer.Methods Thirty patients with bladder cancer admitted to this hospital from September 2017 to September 2019 were selected as the bladder cancer group,and 30 healthy patients during physical examination during the same period were selected as the health group.The serum levels of AHSG and CEA in the two groups were detected by enzyme-linked immunosorbent assay(ELISA)and compared.The expression level of AHSG mRNA in bladder cancer tissues was analyzed by starBase and Oncomine database.The expression level of AHSG protein in bladder cancer tissues was verified by the Human Protein Atlas(HPA)database.The value of AHSG for predicting the prognosis in the patients with bladder cancer was analyzed by using the Kaplan-Meier Plotter(KM Plotter)database.The diagnostic value of AHSG and CEA combined detection for bladder cancer was analyzed by the receiver operating characteristic(ROC)curve.Results The starBase and Oncomine databases analysis found that the expression level of AHSG mRNA in bladder cancer increased significantly,and was closely correlated with the tumor grade,distant metastasis and lymph node metastasis(P<0.05).The HPA database verified that the expression level of AHSG protein in bladder cancer tissue was higher than that in normal bladder tissue.The ELISA results showed that the serum levels of AHSG and CEA in the bladder cancer group were significantly higher than those in the health group(P<0.05).The KM Plotter database analysis found that the bladder cancer patients with high AHSG expression had a better relapse free survival probability than those with low AHSG expression.The ROC curve analysis showed that the area under the curve for the combined detection of AHSG and CEA for the diagnosis of bladder cancer was 0.952,the sensitivity was 90.0%,and the specificity was 93.3%.It is more valuable than AHSG and CEA alone for the diagnosis of bladder cancer.Conclusion The expression of AHSG is significantly increased in the patients with bladder cancer,and it is an objective evaluation indicator for bladder cancer diagnosis and prognosis prediction.It and CEA are potential serological markers with higher clinical application value in the diagnosis and prognosis analysis of bladder cancer.