微小RNA-141-3p通过靶向下调磷酸酶与张力蛋白同源物促进食管鳞状细胞癌细胞的恶性进展

目的揭示微小RNA(miR)-141-3p和磷酸酶与张力蛋白同源物(PTEN)对食管鳞状细胞癌细胞(ESCC)增殖、迁移、侵袭和凋亡的作用以及两者间存在的调控关系。方法利用实时逆转录聚合酶链反应(qRT-PCR)和蛋白质免疫印迹法分别检测ESCC细胞中miR-141-3p和PTEN信使RNA(messenger RNA,mRNA)的表达水平,以及PTEN蛋白表达水平。Starbase数据库预测miR-141-3p的下游靶基因为PTEN,并通过双荧光素酶实验验证二者的靶向关系。分别通过细胞增殖实验(CCK8)实验、Transwell、划痕实验和流式细胞术检测ESCC细胞的增殖、侵袭和迁移能力以及凋亡情况。结果miR-141-3p在ESCC细胞中显著高表达,而PTEN则作为miR-141-3p的直接靶标在ESCC细胞中显著低表达。下调miR-141-3p的表达能够对ESCC细胞的增殖、迁移和侵袭起到抑制作用并促进其凋亡。过表达PTEN能显著减弱miR-141-3p对细胞恶性进展产生的影响。结论miR-141-3p通过靶向调控PTEN的表达,进而促进ESCC细胞的增殖、迁移和侵袭能力,并抑制细胞凋亡。

MiR-141-3p promotes the malignant progression of esophageal squamous cell carcinoma by targeted down-regulation of PTEN

Objective To investigate the effects of miR-141-3p and PTEN on the proliferation,migration,invasion and apoptosis of esophageal squamous cell carcinoma(ESCC)and whether there is a regulatory relation-ship between the two.Methods Real-time quantitative polymerase chain reaction(qRT-PCR)and Western blot-ting were used to examine the expression levels of miR-141-3p and PTEN mRNA and the protein expression lev-els of PTEN in ESCC cells.The Starbase database was used to predict PTEN as the target gene of miR-141-3p in ESCC.The targeting relationship between miR-141-3p and PTEN was verified via dual luciferase assay.The pro-liferation,invasion and migration and apoptosis of ESCC cells were examined by CCK8 assay,Transwell assay,scratch assay and flow cytometry.Results In ESCC cellsmiR-141-3p was significantly highly expressed,while PTEN as a direct target of miR-141-3p was low-expressed.Down-regulating the expression of miR-141-3p can in-hibit the proliferation,migration and invasion of ESCC cells and promote the cell apoptosis.Overexpression of PTEN can significantly reduce the effect of miR-141-3p on the malignant progression of cells.Conclusion In summary,miR-141-3p can improve the proliferation,migration and invasion of ESCC cells and inhibit cell apopto-sis through targeted regulation of PTEN.