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| WIN 55,212-2重复预处理对大鼠局灶性脑缺血再灌注损伤的保护作用 | |
| 引用本文： | 陈烨,蓝天,耿智隆,陈绍洋. WIN 55,212-2重复预处理对大鼠局灶性脑缺血再灌注损伤的保护作用[J]. 解放军医学杂志, 2010, 35(1) |
| 作者姓名： | 陈烨 蓝天 耿智隆 陈绍洋 |
| 作者单位： | 1. 兰州军区兰州总医院麻醉科,兰州,730050 2. 兰州军区兰州总医院泌尿外科,兰州,730050 3. 第四军医大学西京医院麻醉科 |
| 基金项目： | 国家自然科学基金(30572421) |
| 摘 要： | 目的探讨大麻素受体激动剂WIN55,212-2重复预处理对大鼠局灶性脑缺血再灌注损伤的保护作用。方法将50只雄性SD大鼠随机分为5组(n=10):对照组和二甲基亚砜(DMSO)组分别腹腔注射生理盐水和DMSO0.3ml,1次/d,连续5d;WIN55,212-2预处理组(包括WIN1、WIN3、WIN5组)分别腹腔注射WIN55,212-21mg/kg,1次/d,重复给予1、3、5d。各组动物均在最后1次预处理后24h采用大鼠大脑中动脉栓塞(MCAO)法建立短暂局灶性脑缺血模型,分别在MCAO后24、48、72h进行神经功能评分(NFS),并取脑组织切片行2,3,5-氯化三苯四唑(TTC)染色,计算脑梗死容积百分比。结果WIN55,212-2预处理组(WIN1、WIN3、WIN5组)大鼠MCAO后24、48、72h的NFS明显高于DMSO组和对照组(P<0.05),脑梗死容积百分比明显低于DMSO组和对照组(P<0.05)。WIN5组大鼠NFS明显高于脑梗死容积百分比明显低于WIN1组和WIN3组(P<0.05),而WIN1组与WIN3组的NFS和脑梗死容积百分比均无显著性差异(P>0.05)。结论随着WIN... |
| 关 键 词： | 受体 大麻酚 WIN55 212-2 再灌注损伤 预处理 |
Neuroprotective effect of repeated WIN 55, 212-2 preconditioning on focal cerebral ischemia-reperfusion injury in rats |
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| CHEN Ye,LAN Tian,GENG Zhi-long,CHEN Shao-yang. Neuroprotective effect of repeated WIN 55, 212-2 preconditioning on focal cerebral ischemia-reperfusion injury in rats[J]. Medical Journal of Chinese People's Liberation Army, 2010, 35(1) | |
| Authors: | CHEN Ye LAN Tian GENG Zhi-long CHEN Shao-yang |
| Abstract: | Objective To explore the neuroprotective effect of repeated preconditioning with cannabinoid receptor agonist WIN 55,212-2 on focal cerebral ischemia-reperfusion injury in rats.Methods Focal cerebral ischemia was induced by middle cerebral artery occlusion(MCAO)for 120min.Fifty male SD rats were randomly assigned to five groups(10 each):rats in control group and dimethyl sulphoxide group(DMSO group)were intraperitoneally administered 0.3ml normal saline and 0.3ml DMSO once a day for 5 days.Rats in WIN 55,21... |
| Keywords: | receptors cannabinoid WIN 55 212-2 reperfusion injury preconditioning |
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