ROS对内皮细胞胞浆游离钙水平的影响 |
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引用本文: | 林宇,崔红霞,宋娟. ROS对内皮细胞胞浆游离钙水平的影响[J]. 齐齐哈尔医学院学报, 2009, 30(15): 1811-1812 |
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作者姓名: | 林宇 崔红霞 宋娟 |
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作者单位: | 齐齐哈尔医学院药理教研室,161042 |
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基金项目: | 黑龙江省自然科学基金资助 No :ZA2006-05,黑龙江省教育厅科学技术研究项目 NO:11521328 |
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摘 要: | 目的探讨活性氧(ROS)在TNFα刺激人脐静脉内皮细胞(HUVEC)胞浆游离钙水平变化中的作用。方法用氧化-还原敏感的荧光探针2,7-Dichlorofluorescein(DCF)检测细胞内氧化-还原水平。用激光共聚焦显微镜检测胞浆游离钙浓度。结果TNFα(200U/ml)处理24h后,HUVEC胞内ROS水平和游离钙浓度均明显升高;NAC(20mmol/L)预处理可明显抑制胞浆内游离钙浓度(P〈0.01)。结论ROS部分介导了TNFα对胞浆内游离钙水平的诱导作用。
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关 键 词: | 活性氧 内皮细胞 钙 |
Role of ROS on the cytosolic free calcium in vascular endothelial cells |
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Affiliation: | LIN Yu,et al. (Department of pharmacology, Qiqihar Medical University, Qiqihar, Heilongjiang 161042 ,China) |
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Abstract: | Objective To investigate the roles of reactive oxygen species(ROS) on the eytosolic free calcium(FECa^2+]i) in the endothelial cells stimulated by TNFα. Methods The ROS production in the cells was measured by 2,7 - Dichlorofluorescein(DCF) diacetate as probe; The cytosolic free calcium (F[Ca^2+ -]i) was detected by confocal fluorescence microscope. Results TNFα(200 U/ml) treatment caused an increase in the cytosolic free calcium concentration and the ROS production in HUVECs, which was markedly reduced by pretreatment with NAC(20 mmol/L). Conclusions ROS may play a major role in the TNFα-induced upregulation of F[Ca^2+]i. |
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Keywords: | ROS Endothelial cells Ca2+ |
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