| HPLC测定柔肝胶囊中5种活性成分的含量 |
| |
| 引用本文： | 束艳,宿树兰,钱大玮,段金廒,蒋海峰.HPLC测定柔肝胶囊中5种活性成分的含量[J].中国实验方剂学杂志,2010,16(18):71-75. |
| |
| 作者姓名： | 束艳 宿树兰 钱大玮 段金廒 蒋海峰 |
| |
| 作者单位： | 南京中医药大学,江苏省方剂研究重点实验室,南京,210046 |
| |
| 摘 要： | 目的:建立柔肝胶囊含量测定的质量控制方法。方法:应用HPLC-DAD法测定其中丹酚酸B、姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素的含量。采用Apollo C18柱(4.6 mm×250 mm,5μm),以4%醋酸水溶液和乙腈为流动相,梯度洗脱,流速为1.0 mL.min-1,柱温30℃,检测波长286,426 nm。应用HPLC-ELSD法测定其中牛磺熊去氧胆酸的含量。采用A-pollo C18柱(4.6 mm×250mm,5μm),以乙腈-乙酸-乙酸铵缓冲液(pH 4.5)为流动相,等度洗脱,流速为0.8 mL.min-1,ELSD漂移管温度80℃,雾化气体压力35 psi,喷雾器温度36℃。结果:丹酚酸B、姜黄素类成分、牛磺熊去氧胆酸的线性关系良好(r=0.999 3～0.999 9),线性范围分别为0.148 0～1.850,0.125 6～1.570,0.043 2～0.540 0,0.033 6～0.420 0,0.972 0～12.15μg;精密度、重复性、稳定性的RSD都低于3%;加样回收率为97.8%～101.7%。结论:本方法简便快捷、结果准确且重现性好,可用于柔肝胶囊的质量控制。
|
| 关 键 词： | 柔肝胶囊 丹酚酸B 姜黄素类成分 牛磺熊去氧胆酸 高效液相色谱法 含量测定 |
| 收稿时间： | 2010/8/18 0:00:00 |
HPLC Determination of Five Active Substances in Rougan Capsule |
| |
| SHU Yan,SU Shu-lan,QIAN Da-wei,DUAN Jin-ao and JIANG Hai-feng.HPLC Determination of Five Active Substances in Rougan Capsule[J].China Journal of Experimental Traditional Medical Formulae,2010,16(18):71-75. |
| |
| Authors: | SHU Yan SU Shu-lan QIAN Da-wei DUAN Jin-ao and JIANG Hai-feng |
| |
| Institution: | Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, China |
| |
| Abstract: | |
| |
| Keywords: | Rougan capsule salvianolic acid B curcumin constituents tauroursodexycholic acid HPLC determination |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国实验方剂学杂志》浏览原始摘要信息 |
| 点击此处可从《中国实验方剂学杂志》下载免费的PDF全文 |
|
