胃癌组织cyclinE表达的临床病理意义

目的　Ｃｙｃｌｉｎ Ｅ 蛋白在细胞周期的Ｇ１ 晚期起作用，加速Ｇ１Ｓ期转化，与人类肿瘤有关． 本研究探讨ｃｙｃｌｉｎ Ｅ 蛋白在胃癌的意义．方法　用敏感而特异的免疫组化ＳＰ 法测定胃癌石蜡切片中ｃｙｃｌｉｎ Ｅ蛋白的异常表达．结果　Ｃｙｃｌｉｎ Ｅ 在胃癌中表达６２ ％ （ｎ ＝ ６０ ，Ｍ＝ ４３ ，Ｆ＝ １７ ，３２岁～７３ 岁） ，与癌旁组织２６ ％ 相比有显著差异（ Ｐ＜ ０－０５） ．Ｃｙｃｌｉｎ Ｅ表达与组织病理分化程度呈负相关． 分化差的胃癌组织ｃｙｃｌｉｎ Ｅ蛋白表达越强，ｃｙｃｌｉｎ Ｅ与肿瘤侵袭、淋巴结转移、远处转移无明显关系．结论　Ｃｙｃｌｉｎ Ｅ 蛋白与胃癌发生有关，与胃癌进展无关．ＣｙｃｌｉｎＥ 蛋白可做为胃癌的一个潜在的诊断和判断预后的指标．

Cyclin E expression in gastric carcinoma and its clinicopathological significance

AIM Cyclin E protein acts in late G 1 of cell cycle and accelerates the G 1 to S transition, cyclin E is associated with the human cancer. The aim of this study was to investigate the significance of the cyclin E protein expression in gastric carcinoma. METHODS Cyclin E protein overexpression was studied in paraffin embedded sections of gastric carcinoma by means of a specific and sensitive immunohistochemical SP assay. RESULTS Cyclin E protein expression was present in 62% (37/?60) gastric carcinoma, which showed a significant difference from those of normal tissues adjacent to tumors (26%) ( P <0 05). Cyclin E expression was negatively correlated with histological differentiation. Gastric carcinoma with poor differentiation showed increased cyclin E expression, cyclin E was not related to the invasion of tumor, lymph node and distant metastasis. CONCLUSION Cyclin E is involved in the genesis of gastric carcinoma and not contributes to its development. Cyclin E protein is a potential diagnostic and prognostic marker for gastric cancer.