树状DNA杂交技术在HCV检测中的应用

目的：建立一种敏感性，特异性，重复性较好的，适同原体群体筛查的检验方法。方法：通过RT－PCR－DDH，RT－nested-PCR和HCV RNA－DDH3种方法检测HCV核酸，推断RT－PCR－DDH技术检测病毒核酸的特异性，敏感性和稳定性。结果：47份ELISA检测HCV抗体阳性血清标本，RT－nested-PCR检出阳性标本33例（70．21％），RT－PCR－DDH检出阳性标本39例（82．98％），结论：RT－PCR－DDH技术在逆转录病毒核酸检测的应用中具有较好的特异性，敏感性和稳定性，而且成本较低，操作安全简便，适于中小实验室和基层医院病原微生物的检测。

The application of DDH technology in HCV detection

Objective To set up a more sensitive,specific,duplicative method which should adapt to screen pathogenic microorganism in population.Methods By RT PCR DDH,RT nested PCR,RNA DDH,we detect the nucleic acid of HCV and deduce the specificity,sensitivity and stability of RT PCR DDH in viral nucleic acid detection.Result In 47 serum samples which were HCV positive in ELISA,and 33(70.21%) were positive in RT nested PCR,39(82.98%) were positive in RT PCR DDH.Conclusion RT PCR DDH has higher specificity,sensitivity and stability in retroviral detection.Moreover,low cost and easy,safe to operate make it is suitable for the test of pathogenic microorganisam in middle and small laboratories and primary hospitals.