| 骨髓增生异常综合征的-7/7q-染色体异常的检测 |
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| 引用本文： | 申咏梅,薛永权,李建勇,过宇,潘金兰,吴亚芳.骨髓增生异常综合征的-7/7q-染色体异常的检测[J].中华医学遗传学杂志,2001,18(4):255-258. |
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| 作者姓名： | 申咏梅 薛永权 李建勇 过宇 潘金兰 吴亚芳 |
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| 作者单位： | 苏州大学附属第一医院、江苏省血液研究所, |
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| 摘 要： | 目的 探讨间期荧光原位杂交(fluorescence
in situ hybridization,FISH)技术在检测骨髓增生异常综合征(myelodysplastic
syndrome,MDS)-7/7q-染色体异常中的价值。方法 同时应用常规细胞遗传学(conventional
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| 关 键 词： | 骨髓增生异常综合征 荧光原位杂交 染色体异常 细胞遗传学 |
| 修稿时间： | 2000年8月20日 |
Detection of monosomy 7 or 7q- in cases of myelodysplastic syndrome |
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| SHEN Yongmei,XUE Yongquan,LI Jiangyong,GUO Yu,PAN Jinlan,WU Yafang..Detection of monosomy 7 or 7q- in cases of myelodysplastic syndrome[J].Chinese Journal of Medical Genetics,2001,18(4):255-258. |
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| Authors: | SHEN Yongmei XUE Yongquan LI Jiangyong GUO Yu PAN Jinlan WU Yafang |
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| Institution: | First Affiliated Hospital of Suzhou University, Jiangsu Institute of Hematology, Suzhou, Jiangsu 215006 P.R.China. uujihsmc@public1.sz.js.cn |
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| Abstract: | Objective To investigate the value of interphase fluorescence in situ hybridization(FISH) in the detection of monosomy 7 (-7)or deletion of long arm of chromosome 7(7q-) in the cases of myelodysplastic syndrome (MDS). Methods Forty six cases of MDS and 10 normal controls were studied simultaneously by conventional karyotype analysis and interphase FISH technique using SpectrumRed directly labeled DNA specific probe for 7q32. Two hundred interphase cells were analyzed for each case and the cells with one red hybridization spot>7% were regarded as positive. Results Three cases displayed -7/7q- by conventional cytogenetics(CC) and were confirmed by interphase FISH. Six cases in 43 cases who did not show -7/7q- by CC displayed -7/7q- by interphase FISH. Conclusion Interphase FISH is very useful for the detection of -7 or 7q- in MDS and it is more sensitive than CC. |
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| Keywords: | myelodysplastic syndrome fluorescence in situ hybridization chromosome abnormality cytogenetics |
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