膝骨痹康胶囊对膝骨性关节炎大鼠IL-6、IL-9影响的实验研究

目的探讨膝骨痹康胶囊对大鼠膝骨性关节炎（KOA）模型关节软骨中白细胞介素6（IL-6）和白细胞介素9（IL-9）水平的影响。方法选取60只清洁级SD大鼠,从中随机取10只作为空白对照组,其余采用单侧跟腱切除法制造大鼠KOA模型,将符合模型标准的大鼠随机分为五组：模型组,抗骨质增生胶囊组,膝骨痹康胶囊低、中、高剂量组。经药物干预30 d后,采用免疫组织化学方法观察膝关节软骨中IL-6和IL-9的表达。结果关节软骨中IL-6和IL-9的表达空白对照组与模型组比较差异有统计学意义（P〈0.01）;膝骨痹康胶囊中剂量组能显著下调关节软骨中IL-6和IL-9的表达,与模型组比较差异有统计学意义（P〈0.01）。结论膝骨痹康胶囊能够减轻KOA关节软骨破坏及关节病损,其可能的机制是通过减少炎症细胞因子IL-6和IL-9的破坏作用来实现。

Experimental Research of the Impacts of Xigubikang Capsules on IL-6 and IL-9 for the Rats with Knee Osteoarthritis

Objective To explore the impacts of xigubikang capsules on the levels of interleukin-6(IL-6)and interleukin-9(IL-9)in the joint cartilage of the rat models with knee osteoarthritis(KOA).Methods 60 clean-grade SD rats were selected.10 rats of them were randomized to be in a blank control group.The rest rats were prepared as KOA models with unilateral Achilles tendon resection.The rats in compliance with the model standard were randomized into 5 groups,a model group,a kangguzhizengsheng capsules group,a low-dose xigubikang capsules group,a middle-dose xigubikang capsules group and a high-dose xigubikang capsules group.In 30-day medicine intervention,the immunohistochemistry(IHC)method was adopted to observe the expressions of IL-6 and IL-9 in the knee joint cartilage.Results The difference in the expressions of IL-6 and IL-9 in the joint cartilage was significant statistically between the blank control group and the model group(P<0.01).The expressions of IL-6 and IL-9 in the joint cartilage were down-regulated significantly in the middle-dose xigubikang capsules group,presenting the statistical significant difference as compared with that in the model group(P<0.01).Conclusion Xigubikang capsules alleviates the joint cartilage destruction and the joint damage in KOA,which is achieved probably through reducing the destruction of inflammatory cellular factors IL-6 and IL-9.