硒和维生素E对饲予克山病病区粮大鼠肝脏自由基代谢的影响

饲予克山病病区粮对大鼠肝脏自由基代谢的影响及补充硒和维生素E的预防作用研究结果显示:病区粮组动物肝脏谷胱甘肽过氧化物酶活性明显下降,脂质过氧化物含量和自由基水平显著升高。在病区粮饲料中分别加入100ppmDL-α-生育酚和0.22ppm亚硒酸钠,均可明显降低脂质过氧化物含量和自由基水平。病区粮组动物肝脏α-生育酚和还原型谷胱甘肽含量均明显增加,提示硒缺乏可增加其对维生素E的需要量和使还原型谷胱甘肽生成增多。结果表明,克山病病区粮食中的致病因素能引起动物肝脏自由基代谢的紊乱,硒和维生素E对其都有很好的预防作用,提示维生素E的相对不足也有可能在克山病发病机制中起重要作用。

EFFECTS OF SELENIUM AND VITAMIN E ON FREE RADICALS METABOLISM IN LIVER OF RAT FED GRAINS FROM A KESHAN DISEASE ENDEMIC AREA

The changes ot free radicals matebolism in liver of rat fed grains from a Keshan disease endemic area and the effects of selenium and vitamin E on those were studied. The endemic grains that were deficient in selenium caused markedly decrease in the glutathione peroxidase activity, and marked increase in the lipid peroxides content and the free radicals level of the rat livers. The diets that supplemented the endemic grains with either selenium (0.22 mg/kg diets) or DL-alpha-tocopherol (100 mg /kg diets) caused the falls in the lipid peroxides content and the free rdicaals level. There was a marked rise in the al pha-tocopherol content and reduced glutathione content in livers of rats fed grains from the endemic area, suggesting an increased reqiu-rement of vitamin E and production of glutathione in the selenium-defi-cient rats. The results indicate that a disorder of free radicals metaboli- sm is induced by the pathogenic factors existing in the grains cultiva-ted in a Keshan disease endemic area, and is reduced by supplementing the grains with sodium selenium or vitamin E, suggesting that relative lack of vitamin E may play an important role in the mechanism of Keshan disease onset.