高压氧治疗对一氧化碳中毒后迟发性脑病大鼠海马组织中炎症因子表达的影响

目的 研究高压氧(HBO)治疗对一氧化碳中毒(COP)后迟发性脑病(DEACMP)大鼠海马组织中炎症因子表达的影响.方法 将60只雄性SD大鼠随机分为空白对照组(NC组)、迟发性脑病组(DEACMP组)、高压氧治疗组(HBO组),每组16只,另12只备用补充.Morris水迷宫检测各组大鼠学习记忆能力;HE染色法观察各...

Effects of hyperbaric oxygen therapy on the expression of inflammatory factors in hippocampus of rats with delayed encephalopathy after carbon monoxide poisoning

Objective To study the effects of hyperbaric oxygen(HBO)on the expression of inflammatory factors in the hippocampus of rats with delayed encephalopathy after acute carbon monoxide poisoning(DEACMP).Methods 60 male SD rats were randomly divided into normal control(NC)group,DEACMP group and HBO group.There were 16 rats in each group,and the other 12 were used for supplement.Morris water maze was used to evaluate the abilities of learning and memory.HE staining was used to observe the morphological changes and evaluate the grade of damage of hippocampal neurons.The expression level of mitogen-activated protein kinases(MAPK)associated protein,p38 MAPK and p65 nuclear factor kappa-B(NF-κB)were inspected by Western blotting.The expressions level of interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in hippocampus was detected by enzyme-linked immunosorbent assay.Results(1)Compared with NC group,the learning ability of DEACMP group and HBO group decreased in different degrees at 7 d,14 d and 20 d(F=418.300,359.700,530.300,P<0.01).The learning ability of HBO group was higher than that of DEACMP group(P<0.05).On the 20 th day,the memory ability of DEACMP group and HBO group decreased to varying degrees(F=34.690,P<0.01),and the memory ability of HBO group was higher than DEACMP group(P<0.05).(2)Compared with NC group,the hippocampal neurons in DEACMP group and HBO group were damaged in different degrees.The arrangement of neurons and the cytoplasm were loose.Some neurons were necrotic and apoptotic.The degree of damage in HBO group was lighter than that in DEACMP group.(3)The expression levels of p38 MAPK and p65 NF-κB in the hippocampus in DEACMP and HBO group were higher than those in NC group(F=547.800,982.500,P<0.01).The expression levels of p38 MAPK and p65 NF-κB in the hippocampus in HBO group were lower than those in DEACMP group(P<0.05).(4)The expression levels of IL-1β,TNF-αand IL-6 in the hippocampus in DEACMP and HBO group were higher than those in NC group(F=55.200,96.200,81.790,P<0.01).The expression levels of IL-1β,TNF-αand IL-6 in the hippocampus in HBO group were lower than those in DEACMP group(P<0.05).Conclusion After carbon monoxide poisoning,neurons in hippocampus of rats were damaged in different degrees,which may be related to the expressions of p38 MAPK and p65 NF-κB and inflammatory factor,such as IL-1β,TNF-αand IL-6.Hyperbaric oxygen therapy could reduce the damage of neurons in hippocampus and improve the abilities of learning and memory in DEACMP rats,which may be associated with the regulation of MAPK/NF-κB pathway and the reduction of expression levels of inflammatory factors.