运动对生长发育期高脂饲养大鼠棕色脂肪组织功能调控相关基因表达的影响

目的:探讨运动对生长期高脂饲养大鼠棕色脂肪组织功能调控相关基因表达的影响,为少年儿童肥胖的运动防治提供理论依据.方法:54只3周龄SPF清洁级雄性SD大鼠,适应性喂养3天后,随机选取6只大鼠作为基线组(幼儿期)宰杀,其余大鼠随机分为标准安静组(C)、标准运动组(CE)、高脂安静组(HC)、高脂运动组(HE),每组12只...

Effects of Exercise on mRNA Expression of Brown Adipose Tissue Function-regulated Genes in Rats with High-fat Diet during the Growth Period

Objective To explore the effect of exercise on mRNA expression of brown adipose tissue function-regulated genes in rats with high-fat diet during the growth period,so as to provide theoretical basis for the prevention and treatment of obesity for children through exercise.Methods Fifty four three-week-old SPF clean-grade male Sprague-Dawley rats were fed adaptively for 3 days.Then six rats were randomly selected as the baseline group(early childhood) for slaughter,and the remaining rats were randomly divided into a control(C) group,a control exercise(CE) group,a high-fat control(HC) group and a high-fat exercise(HE) group,each of twelve.Rats of the C and CE groups were fed normal diet,while those in the H and HE groups were fed high-fat diet.Rats of the CE and HE groups underwent 60%-70% VO_2max intensity treadmill training(5 days/week,1 hour/day) after threeday adaptive training.Six rats of each group were randomly selected and killed at the age of three weeks(infant),six weeks(pre-puberty),and eight weeks(late-puberty).The mRNA expression of bone morphogenetic protein-4(BMP-4),peroxisome proliferator-activated receptor gamma(PPAR-γ),peroxisome proliferative activated receptor-γ coactivator-1α(PGC-1α),uncoupling protein 1(UCP-1)of brown adipose tissues were tested.Three-way ANOVA was used to measure and compared the results of different groups at different times.Results The body weight and length of all rats increased gradually as they grew and developed.The body weight and length of rats in the 6 wk high-fat diet group were significantly higher than those in the standard group(P<0.05),and those of the 8 wk CE group were significantly lower than the C group.The average energy intake of the high-fat group was higher than that of the standard group(P<0.01).During the growth and development,in the C group,the average mRNA expression of BMP-4,PPAR-γ and UCP-1 in brown adipose tissues decreased,but that of PGC-1α increased significantly(P<0.05).Compared with group C of the same age,the HC group increased significantly in the average mRNA expression of BMP-4 and UCP-1(P<0.01),and the CE group did only in that of BMP-4(P<0.01).Compared with the HC group of the same age,HE group had a significant increase in the average mRNA expression of BMP-4(P<0.01),but a significant decrease in that of PGC-1α and UCP-1 at the age of six weeks(P<0.01).Conclusion Growth and development inhibit the expression of thermogenesis related genes of brown adipose tissues,while high-fat diet promotes their expression.The regulation of exercise on the thermogenesis of brown adipose tissue is affected by the energy state of rats.Therefore,adolescence is a critical period in the regulation of brown adipose tissue thermogenesis in rats.