人类白细胞抗原DRB1基因与肺结核的相关性研究

目的探讨HLA-DRB1基因多态性与肺结核(PTB)的遗传关联性及其与临床表现的关系。方法采用聚合酶链反应-序列特异性引物(PCR-SSP)方法，对74例PTB患者及90名正常人的HLA-DRB1等位基因进行分型。结果与对照组相比，PTB病例组的DRB1*15等位基因频率显著增高(34.3%比17.0%，Pc<0.05，RR=2.91)，HLA-DRB1*12基因频率高(15.4%比7.5%)，但统计学上无显著性差异(Pc>0.05)；而DRB1*11基因频率显著低于对照组(1.4%比9.9%，Pc<0.05，RR=0.12)。HLA-DRB1*15阳性患者中复治病例数和耐药病例数均显著高于HLA-DRB1*15阴性组(P<0.05)。结论HLA-DRB1*15可能是PTB的易感基因，DRB1*11可能为保护基因，HLA-DRB1*15基因与PTB临床特征有一定相关性。

Association of HLA-DRB1 genes with pulmonary tuberculosis

Objective To assess the association of HLA DRB1 genes with pulmonary tuberculosis (PTB) and the correlation between HLA DRB1 alleles and clinical manifestations of patients with PTB. Method Alleles at the HLA DRB1 typing were analyzed by polymerase chain reaction with sequence specific primers (PCR SSP) in 74 patients and 90 healthy controls. Results Compared with controls,a significant increased frequency of DRB1*15 was found (34 3% vs 17 0%, Pc <0 05,RR=2 91) The frequency of DRB1*12 was also increased,but the difference was not statistically significant(15 4% vs 7 5%, Pc >0 05) The frequency of DRB1*11 was significantly decreased (1 4% vs 9 9%, Pc <0 05,RR=0 12) More retreated and drug resistant cases were found in DRB1*15 positive patients than in DRB1*15 negative patients. Conclusions These results show that HLA DRB1*15 allele is susceptible gene in PTB,DRB1*11 may be a protective allele,HLA DRB1*15 allele may be related to patients with refractory PTB