纳络酮和CPP对大鼠急性脊髓损伤的保护作用

目的：评价阿片受体拮抗剂纳络酮（ＮＬＸ）和兴奋性氨基酸受体拮抗剂３－（２－羧基哌嗪－４－基）丙基－１－磷酸（ＣＰＰ）在脊髓损伤后的治疗效果。方法：以７．５ｇ重物从１０ｃｍ高处落下致伤大鼠Ｔ１０脊髓，伤后６０ｍｉｎ开始治疗，从鞘内给予ＮＬＸ１０ｍｇ／ｋｇ、ＣＰＰ１００ｍｇ／ｋｇ、甲基强的松龙（ＭＰ）３０ｍｇ／ｋｇ。结果：ＮＬＸ、ＣＰＰ、ＭＰ均显著促进了脊髓损伤后的神经功能恢复，减少组织损害，ＮＬＸ、ＣＰＰ和ＭＰ之间无显著差别，但较三者合用的效果为差。结论：脊髓损伤后的继发性损伤是多水平、多因素的综合作用，ＮＬＸ、ＣＰＰ与ＭＰ联合应用治疗急性脊髓损伤较之单一用药更有效。

Protective effects of naloxone and CPP on spinal cord injury in rats

To evaluate the effects of naloxone(NLX), an opiate antagonist, and CPP, an excitatory amino acid receptor antagonist in treating spinal cord injury. Methods: An impact of a 7.5 g weight dropped from 10 cm high was used to traumatize the T10 spinal cord. Treatment was started at 60 min posttrauma by intrathecal injection. The naloxone-treated rats received 10 mg/kg bolus, the CPP-treated rats 100 mg/kg, the methylprednisolone(MP)-treated rats 30 mg/kg. Results :NLX, CPP and MP all significantly improved neurological recovery and reduced tissue damage. There were not difference among them(P>0. 05). Effect of combined NLX, CPP and MP was better than that of NLX, CPP or MP alone. Conclusion: The secondary spinal cord injury posttrauma may be multi-level, multiple and composite factors.Beneficial actions of NLX and CPP, in combination with MP make them candidates for use in future clinical treatment.