改良双套管法大鼠原位肝移植术

目的制作改良双套管法大鼠原位肝移植模型。方法在原二袖套法的基础上，于受体门静脉和肝下下腔静脉吻合前放出部分高凝血液，而肝上下腔静脉的吻合是供肝膈肌环与受者肝上下腔静脉血管之间进行（降落伞式吻合），胆道处理的改进和受体肝脏切除时排出肝脏内血液保证受体的有效血容量。结果改良双套管法吻合效果确实可靠，手术成功率达95％，动物的周存活率为97％。结论该模型稳定可靠，可用于大鼠肝移植方面的研究。

Midification of Two-cuff Technique Orthotopic Liver Transplantation Model

Objective To develop an advanced two-cuff technique orthotopic liver transplantation model in rats.Methods On the basis of the previous two-cuff technique,some hypercoagulable blood was released from the recipient's PV and IVC before the cuffed donor PV and IVC were inserted into the lumen of the recipient's PV and IVC.The anastomosis of recipient's SVC was between the donor's diaphragm venaccaval hiatus and recipient's SVC(in parachute model).The improvement in dealing with the gallbladder tract and the discharging the blood in the recipient'liver at the time of the excision were to ensure the recipient systemetic blood capability.Results Every anastomosis was effective.The operative successful rate and one-week survival rate were 95.0% and 97.0% respectively.Conclusion This model could be used as an effective measure for the study of liver transplantation in rats.