| 伴生菌对猪苓几种酶活性的影响 |
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| 引用本文： | 邢晓科,郭顺星. 伴生菌对猪苓几种酶活性的影响[J]. 中国中药杂志, 2004, 29(4): 310-313 |
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| 作者姓名： | 邢晓科 郭顺星 |
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| 作者单位： | 中国协和医科大学,中国医学科学院,药用植物研究所,北京,100094 |
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| 基金项目： | 国家重大科技专项“创新药物和中药现代化”基金资助项目，国家杰出青年科学基金专项( 3 0 3 2 5 0 47) |
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| 摘 要： | 目的 :研究猪苓Grifolaumbellata在与伴生菌Companionfungus共培养过程中 ,伴生菌对猪苓几种酶的活性影响。方法 :测定与伴生菌共培养过程中猪苓菌丝几丁质酶、β-1,3-葡聚糖酶、蛋白酶及胞外多酚氧化酶的活性。结果 :伴生菌能诱导猪苓几丁质酶及 β-1,3-葡聚糖酶活性的提高 ;蛋白酶活性没有明显变化 ;液体培养时 ,在培养的后期猪苓与伴生菌共培养的发酵液中两种胞外多酚酶活性均居于猪苓与伴生菌单独培养的酶活性之间。结论 :伴生菌与猪苓的营养互补可能为猪苓提供一些菌核形成的相关物质 ,从而有利于猪苓形成菌核。
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| 关 键 词： | 猪苓 伴生菌 几丁质酶 蛋白酶 多酚氧化酶 |
| 文章编号： | 1001-5302(2004)04-0310-04 |
| 收稿时间： | 2002-08-27 |
Effects of companion fungus on several enzymatic activities of Grofola umbellata |
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| XING Xiao ke;GUO Shun xing. Effects of companion fungus on several enzymatic activities of Grofola umbellata[J]. China Journal of Chinese Materia Medica, 2004, 29(4): 310-313 |
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| Authors: | XING Xiao ke GUO Shun xing |
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| Affiliation: | Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100094, China. |
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| Abstract: | OBJECTIVE: To study the effect of companion fungus on several enzymatic activities of Grifola umbellata. METHOD: Chitinase, beta-1,3-glucanase, proteinase and extracellular enzymes of G. umbellata were measured during dual culturing with companion fungus. RESULT: Companion fungus could induce the increase of chitinase and beta-1, 3-glucanase activities of G. umbellata. noevident changeswere found in proteinase activity. When in liquid culture, the activities of extracellular enzymes in dual cultured filtrate were between of these of G. umbellata and companion fungus in monocultures. CONCLUSION: Sclerotia differentiation related materials supplied by mutual nutritional supplement between G. umbellata and companion fungus conduce to sclerotial formation of G. umbellata. |
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| Keywords: | Grotola umbellata companion fungus chitinase proteinase polyphenol oxidase |
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