鲍温样丘疹病患者HPV E6/E7 mRNA与血清L1-IgG和存活素表达关系

目的 分析人乳头瘤病毒（HPV）感染所致鲍温样丘疹病（BP）患者 L1-IgG抗体、存活素（Survivin）表达水平,为鲍温样丘疹病有效防治提供科学依据。方法 选取2019年1月—2022年1月秦皇岛市第一医院收治的HPV感染致BP患者150例。根据HPV分型分为高危型组112例和非高危型组38例。采用酶联免疫吸附法检测血清HPV L1-IgG抗体、Survivin、免疫球蛋白IgA、IgG、IgM、白介素6（IL-6）、肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）水平;采用流式细胞术检测患者外周血CD3⁺、CD4⁺、CD8⁺和CD4⁺/CD8⁺水平;使用杂交信号方法核酸检测技术,检测标本中HPV E6/E7 mRNA数量;Spearman相关性分析HPV E6/E7 mRNA和L1-IgG抗体、Survivin阳性表达水平关系。结果 高危型组和非高危型组患者L1-IgG和Survivin阳性表达分别为73.21%（82/112）、67.86%(76/112）和26.32%（10/38）、31.58%（12/38）,差异均有统计学意义（χ²=26.314,15.400,P<0.01）。高危型组患者HPV E6/E7 mRNA 为（1 125.64±113.42）copies/mL,且L1-IgG、Survivin阳性表达HPV E6/E7 mRNA水平较高,呈正相关（P<0.05）。高危型组和非高危型组患者IgA、IgG、IgM分别为（1.23±0.13）、（8.89±0.91）、（1.13±0.12）g/L和（1.60±0.17） 、（11.42±1.15） 、（1.53±0.16）g/L,高危型组较低,差异均有统计学意义（t=13.971,13.814,16.246,P<0.05）。高危型组和非高危型组患者患者CD3⁺、CD4⁺分别为（58.78±5.91）%、（29.96±3.12）%和（65.48±6.61）%、（65.48±6.61）%,高危型组较低,差异均有统计学意义（t=5.858,8.249,P<0.01）。两组患者CD8⁺和CD4⁺/CD8⁺水平比较,差异无统计学意义（P>0.05）。高危型组患者TNF-α、IFN-γ和IL-6水平分别为（2.64±0.30） 、（4.36±0.53）和（3.11±0.32） pg/mL,高于非高危型组患者（1.67±0.18）、（2.44±0.25） 和（2.21±0.25）pg/mL,差异均有统计学意义（t=8.792,21.499,15.769,P<0.05）。结论 BP患者感染HPV可影响患者免疫调节功能和炎症反应,且HPV高危型患者L1-IgG抗体和Survivin阳性表达水平高于非高危型组患者。

Relationship between HPV E6/E7 mRNA and serum L1 IgG antibody,Survivin in patients with Bowenoid papulosis

Objective To analyze the expression levels and significance of L1-IgG antibody and Survivin in patients with Bowenoid papulosis (BP) induced by human papillomavirus (HPV) infection, and to provide scientific basis for effective prevention and treatment of Bowenoid papulosis. Methods A total of 150 patients with BP induced by HPV infection admitted to Qinhuangdao First Hospital were enrolled between January 2019 and January 2022. According to different HPV types, they were divided into high-risk group (n=112) and non-high-risk group (n=38). The levels of serum HPV L1-IgG antibody, Survivin, immunoglobulins (IgA, IgG, IgM), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) were detected by enzyme-linked immunosorbent assay. The levels of peripheral blood CD3⁺, CD4⁺, CD8⁺ and CD4⁺/CD8⁺ were detected by flow cytometry. The number of HPV E6/E7 mRNA in samples was quantitatively detected by hybridization signal method nucleic acid test. The relationship between HPV E6/E7 mRNA and L1-IgG antibody, Survivin was analyzed by Spearman correlation analysis. Results The positive expression levels of L1-IgG antibody and Survivin in high-risk group were 73.21%（82/112）and 67.86%(76/112）, higher than those in non-high-risk group [26.32%（10/38）, 31.58%（12/38） (χ²=26.314,15.400,P<0.05). HPV E6/E7 mRNA in high-risk group was (1 125.64±113.42) copies/mL. In high-risk group, expression level of HPV E6/E7 mRNA was higher in patients with positive expressions of L1-IgG antibody and Survivin, showing positive correlation (P<0.05). The levels of IgA, IgG and IgM in high-risk group were (1.23±0.13) g/L, (8.89±0.91) g/L and (1.13±0.12) g/L, lower than those in non-high-risk group [(1.60±0.17) g/L, (11.42±1.15) g/L, (1.53±0.16) g/L] (t=13.971, 13.814,16.246, P<0.05), and levels of CD3⁺ and CD4⁺ [(58.78±5.91)%, (29.96±3.12)%] were lower than those in non-high-risk group [(65.48±6.61)%, (65.48±6.61)%] (t=5.858, 8.249, P<0.05). There was no significant difference in levels of CD8⁺ and CD4⁺/CD8⁺ between the two groups (P>0.05). The levels of TNF-α, IFN-γ and IL-6 in high-risk group were (2.64±0.30) pg/mL, (4.36±0.53) pg/mL and (3.11±0.32) pg/mL, higher than those in non-high-risk group [(1.67±0.18) pg/mL, (2.44±0.25) pg/mL, (2.21±0.25) pg/mL] (t=8.792,21.499, 15.769, P<0.05). Conclusions HPV infection will affect immune function and inflammatory response in BP patients. The positive expression levels of L1-IgG antibody and Survivin in patients with high-risk HPV infection are higher than those with non-high risk.