| BDNF和红景天甙对体外神经干细胞向GABA能神经元分化的研究 |
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| 引用本文： | 和梅,张瑶,李莹,杨慧.BDNF和红景天甙对体外神经干细胞向GABA能神经元分化的研究[J].黑龙江医药科学,2009,32(4):80-81,82. |
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| 作者姓名： | 和梅 张瑶 李莹 杨慧 |
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| 作者单位： | 1. 佳木斯大学附属第一医院神经内科,黑龙江,佳木斯,154003
2. 佳木斯大学,黑龙江,佳木斯,154007 |
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| 基金项目： | 黑龙江省中医药中西医结合重点科研项目，黑龙江省教育厅科研项目 |
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| 摘 要： | 目的:探讨BDNF和红景天甙对体外神经干细胞向GABA能神经元分化的研究.方法:取新生大鼠海马组织,将培养的神经干细胞分为对照组;实验组:BDNF组、红景天甙组、BDNF和红景天甙组,免疫荧光双标观察并测定Brdu/NSE,Brdu/GAD65阳性分化率.结果:BDNF和红景天甙组Brdu/NSE、Brdu/GAD65阳性分化率14d达最高,其余各组Brdu/NSE阳性分化率7d达最高;BDNF组Brdu/GAD65阳性分化率7d最高,对照组和红景天甙组无明显变化;BDNF和红景天甙组14d Brdu/NSE、Brdu/GAD65阳性分化率均高于对照组、BDNF组和红景天甙组(P<0.05),且Brdu/GAD65阳性分化率高于本组3、7d(P<0.05).结论:①红景天甙有利于神经干细胞向神经元方向分化;②红景天甙可以提高BDNF对神经干细胞定向分化的能力;③BDNF与红景天甙联合有利于神经干细胞向GABA能神经元分化.
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| 关 键 词： | 神经干细胞 红景天甙 GABA能神经元 |
Role of brain derived neurotrophic factor and Salidroside in promoting in vitro nerve stem cells to the differentiation of GABAergic neurons |
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| HE Mei,ZHANG Yao,LI Ying,YANG Hui.Role of brain derived neurotrophic factor and Salidroside in promoting in vitro nerve stem cells to the differentiation of GABAergic neurons[J].Heilongjiang Medicine and Pharmacy,2009,32(4):80-81,82. |
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| Authors: | HE Mei ZHANG Yao LI Ying YANG Hui |
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| Institution: | 1.The First Affiliated Hospital of Jiamusi University;Jiamusi 154003;China;2.Postgraduate of Grade 2006;Jiamusi University;Jiamusi 154007;China |
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| Abstract: | Objective: To explore the role of the brain derived neurotrophic factor(BDNF) and Salidroside in promoting rat neural stem cells to the differentiation of GABAergic neurons.Methods:The neural stem cells were isolated from the hippocampus of the newborn rats and cultured and were divide into the control group and the experimental groups including the BDNF group,the Solidroside group and the BDNF and Solidroside group,and the immunityfluorescence double maker observation was carried out and the positive diffe... |
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| Keywords: | BDNF |
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