高转移人卵巢癌裸鼠皮下移植瘤模型的建立及其生物学特性

用人卵巢癌细胞系HO－8910移植探鼠，建立高转移人卵巢癌探鼠皮下移植瘤模型命名为（NSMO），已传代23次，仍保持高转移的特性。共接种BALB/c棵小鼠57只，皮下成瘤率为100％，平均带瘤存活时间为159．9天。在解剖47只裸鼠中有转移瘤鼠42只（89．4％）。最早出现转移的时间为56天。移植瘤组织学和超微结构保持原来人卵巢分化差的浆液性乳头状囊腺癌的形态特征和分泌功能。流式细胞术分析显示DNA指数为1．4，染色体分析显示众数为54（属于超二倍体），显示人类癌症的特征。移植瘤相关标记物检测显示大多数癌细胞雌激素受体和孕激素受体阳性。该模型为转移机制的研究和寻找抗转移药物提供了理想的工具。

Establishment and characterization of a model of highly metastatic human ovarian cancer transplanted subcutaneously into nude mice

A model of highly metastatic human ovarlan cancer transplanted subcutaneously intonude mice was established (NMSO). Although the NMSO transplanted tumors were passaged 23 times,they retained their highly metastatic behavior. A total of 57 adult Balc/c nude mice (8-14 weeks old )were inoculated (SC) with tumor, the transplantations were 100% successuful and the average survivalperiod was 159. 9 days. 47 mice were dissected and 42 mice were found to have tnetastatic tumors. Theearliest appearance of metastasls was 56 dys- 18 male nude mice all had metastasis. Histology and ultra structure showed that the rnetastatic tumors retained their malignant features and secreting function ofthe original poorly differentiated human ovarian serous papillary cystadenocarcinoma. FCM analysis gavea 1. 4 DNA index and the chromosome mode number was 54 (hyper-diploid), exhibiting the features ofhuman carcinoma. The detection of correlative label material showed that most cancer cells were ER andPR positive- The use of NMSO model may lead to better understanding of the mechanism of metastasisand help search for anti-metastatic agents.