窒息法致大鼠心脏骤停模型复苏的影响因素

目的 观察心肺复苏（CPR）效果,并探讨窒息时间及CPR时间对大鼠自主循环恢复（ROSC）率和6 h生存率的影响.方法 采用呼气末夹闭气管窒息法,建立大鼠心脏停搏（A）模型.30只Sprague Dawley（SD）大鼠随机分2组,窒息7 min组（n=15）,窒息9min组（n=15）.观察大鼠窒息及心肺复苏期间平均动脉压（MAP）、心电图（ECG）、心率（HR）和动脉血pH值的变化,比较各组的ROSC率和6 h生存率.结果 窒息7 min组ROSC率和6h存活率与窒息9 min组比较,差异无显著性（P＞0.05）;CPR时间＜5 min者6 h生存率显著高于CPR时间＞5 min者（P＜0.05）;存活时间大于6 h者CPR时间与存活时间小于6 h者CPR时间比较有显著性差别（P＜0.05）.结论 该复苏模型制作简单,重复性好,可观察多个指标,能满足心肺复苏实验研究的需要;窒息时间选择应控制在7～9 min以内;CPR时间是预测CPR成功率的重要指标.

Factors influencing asphyxial cardiac arrest

Objective To observe the effect of cardiopulmonary resuscitation(CPR), and to investigate the effect of aspyhxial time and CPR time on return of spontaneous circulation (ROSC) and 6-hour survival rate.Methods The animal model of cardiac arrest(CA) was made by clamping endotracheal tube at expiration. Thirty male Sprague Dawley(SD)rats were randomly divided into two groups: 7-minute asphyxiation group (n=15) and 9-minute asphyxiation group(n=15).Mean arterial pressure (MAP), electrocardiogram (ECG), heart rate (HR) and arterial blood gas were monitored during asphyxia and CPR. The ROSC and 6-hour survival rate were compared between the two groups.Results The ROSC rate and 6-hour survival rate in 7-minute asphyxiation group were not statistically different from those in 9-minute asphyxiation group(P>0.05). 6-hour survival rate was higher in rats whose CPR<5 minutes than that in rats whose CPR> 5 minutes (P<0.05).CPR in rats that survived more than 6 hours was shorter than that in rats surviving less than 6 hours(P<0.05).Conclusion The model is easy to be duplicated Asphyxia time can be selected between 7 and 9 minutes. The CPR time is an important predictor of resuscitation success rate .