| 核酸提取方法对乙肝病毒定量PCR检测结果的影响 |
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| 引用本文： | 黄玥,张卫华,王龙武,徐克前.核酸提取方法对乙肝病毒定量PCR检测结果的影响[J].实用预防医学,2004,11(4):717-718. |
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| 作者姓名： | 黄玥 张卫华 王龙武 徐克前 |
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| 作者单位： | 中南大学医学技术与情报学院医学检验系,中国湖南,长沙,410078 |
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| 摘 要： | 目的寻找简单、快速地提取血清乙型肝炎病毒(HBV)DNA并能应用于定量PCR测定的方法. 方法使用碱裂解法、酚氯仿法和煮沸裂解法同时提取血清HBV-DNA,比较荧光定量PCR测定的重复性和测定值的差异. 结果碱裂解法所得HBV-DNA量高于酚氯仿法和煮沸裂解法(P＜0.05),并且碱裂解法所得结果的重复性好于酚氯仿法和煮沸裂解法(CV分别为0.21,0.28和0.33). 结论碱裂解法提取HBV-DNA简单、快速,适用于HBV-DNA荧光定量测定.
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| 关 键 词： | 乙型肝炎病毒 DNA 提取 PCR |
| 文章编号： | 1006-3110(2004)04-0717-02 |
| 修稿时间： | 2004年3月9日 |
The Influence of Nucleic Acid Extraction from Serum on HBV DNA Detection by Fluorescence Quantitative PCR |
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| HUANG Yue,ZHANG Wei-hua,WANG Long-wu,et al..The Influence of Nucleic Acid Extraction from Serum on HBV DNA Detection by Fluorescence Quantitative PCR[J].Practical Preventive Medicine,2004,11(4):717-718. |
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| Authors: | HUANG Yue ZHANG Wei-hua WANG Long-wu |
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| Abstract: | Objective To develop a simple and rapid method for extracting nucleic acid from serum on HBV-DNA detection by PCR. Methods an Alkaline lysis method was used to isolate serum HBV-DNA for fluorescence quantitative PCR and compared with conventional phenol-chloroform extraction method and boiling lysis method. Results The HBV-DNA quantitative values by PCR using the alkaline lysis were higher than the phenol-chloroform extraction method and boiling lysis method ( P<0.05). Moreover,The HBV-DNA quantitative values by PCR using the alkaline lysis were more reproducible than the phenol-chloroform extraction method and boiling lysis method (CV: 0.21,0.28 and 0.33,respectively). Conclusion The alkaline lysis method is simple,rapid and efficient for extraction of serum HBV-DNA and suitable for HBV-DNA quantitative PCR detection. |
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| Keywords: | HBV DNA Extraction PCR |
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