美洲大蠊不同提取物及不同提取方法对肿瘤细胞的细胞毒性作用

目的:研究美洲大蠊不同提取物及不同提取方法对肿瘤细胞的细胞毒性,为美洲大蠊的抗肿瘤细胞研究提供实验依据。方法:以人肝癌SMMC-7721细胞株,红白血病细胞株K562,人结肠癌细胞株HCT116 3种肿瘤细胞为研究对象,采用细胞计数试剂盒-8(CCK-8)法,比较不同的提取物(乙醇提取液、石油醚部位、乙酸乙酯部位、正丁醇部位)和不同的提取方法(渗漉法、超声法、回流法)对3种肿瘤细胞的体外抗肿瘤活性。结果:美洲大蠊乙醇提取液和乙酸乙酯部位对上述肿瘤细胞增殖均有不同程度的抑制作用,其中以乙醇提取液的活性最强;3种提取方法提取的美洲大蠊均表现出不同程度的体外抗肿瘤活性,其中以渗漉法提取的美洲大蠊对3种肿瘤细胞的抑制作用最强,所计算出的半数抑制浓度(IC50)均最小。结论:美洲大蠊乙醇提取液的体外抗肿瘤活性最强,乙酸乙酯部位次之,美洲大蠊宜选择渗漉法提取。

Tumor Cytotoxicity of Different Extracts and Different Extraction Methods of Periplaneta americana

Objective: To study the tumor cytotoxicity of different extracts and different extraction methods of Periplaneta americana. Method: Cell counting kit-8 (CCK-8) method was used to compare the anti-tumor activities of different extracts (ethanol extract, petroleum ether fraction, ethyl acetate fraction and n-butanol fraction) and different extraction methods (percolation method, ultrasonic method, and reflux method) on three kinds of tumor cells (human liver cancer SMMC-7721 cells, human erythroleukemia K562 cells, and human colon cancer cell line HCT116). Result: The ethanol extract and ethyl acetate fraction of P. americana had inhibitory effect on the proliferation of above tumor cells in varying degrees, and the activity was strongest for ethanol extract; P. americana extracted by three extraction methods showed antitumor activity in vitro in varying degrees, and the percolation method had the strongest inhibitory effect on three kinds of tumor cells with minimal IC₅₀ values. Conclusion: Ethanol extract of P. americana had strongest in vitro antitumor activity, followed by ethyl acetate fraction, and percolation method shall be used for extraction.