间质干细胞移植在大鼠缺血性脑卒中的实验研究

目的:探索间质干细胞移植在脑梗塞大鼠的脑内分化及促进神经功能的修复作用。方法:从健康人的肋骨骨髓中分离、纯化而获得的间质干细胞,经体外培养、扩增、鉴定的同时制造大脑中动脉皮层梗塞的实验动物模型;并且,在梗塞后10 d移植所鉴定的间质干细胞经免疫组化验证间质干细胞的脑内成活、及神经性分化后,在第2周和第6周进行神经功能评分。结果:间质干细胞在梗塞灶周边向神经元和神经胶质细胞的方向分化;移植的神经功能评分,修复作用与对照组有显著差异。结论:人间质干细胞为脑梗塞治疗提供了新的思路。

Therapeutic action of transplantation of marrow mesenchymal stem cells into infarction area after cerebral middle artery occlusion in rats

AIM: To explore the differentiation and the functional behavior of marrow mesenchymal stem cells (MSC) transplanted into the cerebral infarction area after cerebral middle artery ischemia in rats. METHODS: MSC were isolated from human rib marrow and cultured in L DMEM medium in vitro. The model of rat cerebral infarction by cerebra middle artery occlusion was established, and the identified MSC were transplanted intracerebrally 10 days later. Immunohistochemistry technique was used to identify the cell survivor and its differentiation to the neurogenesis in the transplantation site, and at 2 weeks and 6 weeks after transplantation, the functional tests were comparatively studied. RESULTS: The results showed that the survivor of transplanted MSC was differentiated to neural phenotype cells, and the functional behavior of the injury rats was recovered significantly after MSC transplantation (P<0 05). CONCLUSION: Our data suggest that transplantation of MSC may be a powerful autoplastic therapy for the stroke.