卵巢癌血清差异表达蛋白分析

目的：探讨卵巢癌血清差异表达蛋白对提高卵巢癌的诊断的意义。方法：采用弱阳离子（WCX）磁珠纯化试剂盒和基质辅助激光解吸电离飞行时间质谱（MALDI—TOF—MS）技术，对41例晚期卵巢癌（FIGOⅢ、Ⅵ期）、40例良胜卵巢肿瘤患者及40例健康人群对照血清标本进行检测，筛选出卵巢癌患者与对照组血清中的差异表达质谱峰，并建立诊断模型；再用此模型对8例早期卵巢癌（FIGOI、Ⅱ期）进行测试，检测其用于早期卵巢癌诊断的可行性。结果：在Mr1000—12000区段，发现卵巢癌与对照组间差异表达蛋白峰8个（P〈0．01或P〈0．05），其中低表达的4个，Mr分别为1457，1857，2202，7761，高表达的4个，Mr分别为：2946，5333，5859，5901。用它们建立成卵巢癌的诊断模型，对8例早期卵巢癌患者进行预测，7例预测准确，准确率为87．5％。结论：MALDI—TOF—MS结合磁珠技术能直接检测出晚期卵巢癌患者血清差异表达蛋白，并且适用于早期卵巢癌患者的诊断，对提高卵巢癌诊断的敏感性和特异性具有一定的临床意义。

Analysis of differential expressed protein from ovarian cancer serum

Objective：To study the valae of the differential expressed proteins from ovarian cancer serum for early diagnosis of ovarian cancer. Methods ： WCX kit （ Bruker Daltonics GmBH） and matrix - assisted laser desorption/i- onization time - of - fligh - t mass spectrometry technology were used to detect serum samples from 41 patients with advanced ovarian cancer,40 patients with benign ovarian tumor and 40 healthy women for screening differential ex- pressed protein peaks. A diagnostic model was established with these protein peaks. And the nthe diagnostic pattern was applied to 8 patients with early - stage ovarian cancer to investigate the feasibility of diagnosis. Results： In the mass range from 1 000 to 12 000 Da,eight differential expressed protein peaks were screened. Among them,four pro- tein peaks with Mr 1457,1857,2202,7761 were down -regulated and the others with Mr 2946,5333,5859,5901 were up - regulated. A diagnostic pattern consisted of these eight protein peaks was established. The pattern was applied to 8 early - stage ovarian cancer serum samples, and 7 serum samples were successfully predicted with the accuracy of 87.5%. Conclusion ： Combination of MALDI - TOF - MS and WCX kit technology can directly screen the differren- tial expressed protein from ovarian cancer serum, and these protein were suitable to diagnosis of early - stage ovarian cancer. They have clinical significance for enhancement of sensitivity and specificity of ovarian cancer diagnosis.