电针对膝骨关节炎MIA模型大鼠滑膜组织中MMP-3／TIMP-1表达的影响

目的：观察电针与塞来昔布治疗大鼠膝骨关节炎后，大鼠滑膜组织中基质金属蛋白酶-3（ MMP-3）和基质金属蛋白酶抑制剂-1（TIMP-1）含量的变化。方法：选用SD大鼠40只，采用随机数字表随机分成正常组、模型组、药物组和电针组，每组各10只。通过向SD大鼠左侧关节腔内注射单体碘乙酸钠（ MIA）并驱赶大鼠运动来建立膝骨关节炎MIA模型。造模两周后，对电针组取“后三里”穴、“阳陵泉”穴进行电针治疗；对药物组给予塞来昔布灌胃治疗；正常组跟造模组不做任何干预。结果：模型组滑膜组织中MMP-3和TIMP-1的含量较正常组显著提高（ P＜0．05），但MMP-3和TIMP-1的变化不同步；经过两周的治疗，电针组跟药物组滑膜组织中的MMP-3和TIMP-1含量较造模组显著降低（P＜0．05），但仍然略高于正常组。电针治疗组和药物治疗组中两者含量相近（P＞0．05），无统计学意义。结论：电针能有效平衡MIA膝骨关节炎模型大鼠中MMP-3和TIMP-1的异常表达，这可能是电针治疗膝骨关节炎有效的机制之一，为针灸治疗膝骨关节炎提供了有力的证据。

Effect of Electroacupuncture on Matrix Metalloproteinase-3 and Tissue Inhibitor of Metalloproteinase-1 in the Synovium of Knee Osteoarthritis MIA Model Rats

Objective：To observe the changes of MMP -3 and TIMP-1 expression level in synovium of knee osteoarthritis rats after they were treated with electroacupuncture and oral celecoxib .Methods：Forty cases of male SD rats were randomly assigned into four groups ,and these were normal group , model group , electroacu-puncture group and celecoxib group .KOA model was constructed by injecting mono -iodoacetate （ MIA） to left articular cavity .Electroacupuncture group was treated with electroacupuncture at “Yang-Lingquan”（ GB34 ） and “Hou-sanli”（ ST36 ） with a needle afflicted to an electroacupuncture apparatus .Celecoxib group was ga-vaged with a dose of 3mg/（kg -1·2ml -1）.Model group received no treatment.And normal group was estab-lished as blank .Results：MMP-3 and TIMP-1 in synovium of model group were significantly increased （ P〈0 .05 ） compared with that in the normal group in the fourth week after treatment ,and the balance between them was significantly broken;the contents of MMP -3 and TIMP -1 in electroacupuncture group and celecoxib group were reduced compared with that in the model group （ P〈0.05） and they were back to be balance again after treatment.In addition,there was no great difference between two groups （P 〉0.05）.Conclusion：This sduty demonstrates that electroacupuncture can effectively correct the imbalance between MMP -3 and TIMP-1 in synovium of knee osteoarthritis model rats .It may be one of the mechanisms in the treatment of knee osteo-arthritis by electroacupuncture ,which offers powerful support for acupuncture treatment of KOA .