白介素13基因多态性与哮喘发病易感性的关系 |
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引用本文: | 席素雅,;陈树珍,;刘建强,;张玲,;王少颖.白介素13基因多态性与哮喘发病易感性的关系[J].山东医药,2014(29):18-20. |
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作者姓名: | 席素雅 ;陈树珍 ;刘建强 ;张玲 ;王少颖 |
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作者单位: | [1]河北省胸科医院,石家庄050000; [2]河北省老年病医院,石家庄050000; |
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摘 要: | 目的:探讨白介素13(IL-13)基因内含子区+1923C/T多态性与哮喘发病易感性的关系。方法采用聚合酶链反应-限制性片段长度多态性技术对150例哮喘患者(哮喘组)和150例健康对照者(对照组) IL-13基因内含子区+1923 C/T单核苷酸多态性进行检测,比较其基因型和等位基因分布频率。结果对照组IL-13基因内含子区+1923 C/T基因型CC、CT和TT的分布频率分别为41.33%(62/150)、44.00%(66/150)和14.67%(22/150),在哮喘组分别为21.33%(32/150)、41.33%(62/150)和37.34%(56/150),两组各基因型分布频率比较差异有统计学意义(χ^2=24.52,P<0.01)。 CT、TT基因型者患哮喘的危险性高于CC基因型者(χ^2=27.38,P<0.01)。结论 IL-13基因内含子区+1923 C/T多态性是影响哮喘发病的重要候选基因,T等位基因与哮喘易感性相关。
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关 键 词: | 哮喘 白细胞介素13 多态性 单核苷酸 聚合酶链反应-限制性片断长度多态性技术 interleukin-13 |
Correlation between IL-13 gene polymorphism and susceptibility to asthma |
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Institution: | XI Su-ya, CHEN Shu-zhen, LIU Jian-qiang, ZHANG Ling, WANG Shao-ying( 1 Chest Hospital of Hebei Province, Shijiazhuang 050000, China) |
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Abstract: | Objective To investigate the correlation between the single nucleotide polymorphism ( SNP) of +1923 C/T in intron region of interleukin-13 (IL-13) gene and the susceptibility to asthma .Methods The genotypes and the allele frequency distributions of +1923 C/T SNP of IL-13 in 150 asthmatic patients and 150 healthy controls were detected by u-sing polymerase chain reaction-restriction fragment length polymorphism ( PCR-RFLP) methods.Results The genotype frequencies of CC, CT and TT in +1923C/T sites of IL-13 gene were 41.33% (62/150), 44.00% (66/150) and 14.67%(22/150) respectively in the controls, and 21.33%(32/150), 41.33%(62/150) and 37.34%(56/150) in the asthmatic subjects .The distribution of genotype in +1923 C/T sites of IL-13 gene between the two groups was significantly different (χ^2 =24.52, P〈0.01).The CT and TT genotypes carried higher risks for asthma than CC genotype (χ^2 =27.39, P〈0.01).Conclusion The +1923 sites polymorphism of IL-13 gene may be an important candidate gene for asthma and the T allele in the gene might be associated with the susceptibility to asthma . |
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Keywords: | asthma polymorphism single nucleotide polymerase chain reaction-restriction fragment length polymorphism |
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