核桃楸皮化学成分的鉴定及其抗肝癌活性成分筛选

目的：鉴定核桃楸皮的化学成分，并对其进行体外抗肝癌活性成分筛选。方法采用75％乙醇回流法提取核桃楸皮，经分级萃取、硅胶柱层析及制备型高效液相色谱分析等方法得到单体化合物，通过化合物的理化性质及核磁共振技术鉴定化合物成分；采用MTT法评价其体外抗肝癌活性。结果从核桃楸皮中分离得到2种黄酮类化合物，经鉴定分别为木犀草素和刺芒柄花素。 MTT检测结果表明，木犀草素对肝癌细胞HepG2和正常肝细胞LO2都有一定的毒性作用，IC50分别为（103．32±21．34）、（131．13±13．48）μmol/L；刺芒柄花素对肝癌细胞HepG2的毒性作用较小，其IC50＞200μmol/L。结论核桃楸皮含2种黄酮类化合物：木犀草素和刺芒柄花素，其中木犀草素具有明显的抗肝癌作用。

Chemical compositions of Cortex Juglans Mandshurica and their activity screening of anti-hepatocarcinoma effect

Objective To investigate the chemical compositions of Cortex Juglans Mandshurica and to evaluate anti-tumor effects of these compounds in human hepatoma cells.Methods Cortex Juglans Mandshurica were extracted with 75%ethanol, concentrated with chloroform and ethyl acetate, eluted radiantly on silica gel, and purified by preparative HPLC.Physicochemi-cal constants and spectroscopic analysis were used to identify their structures .MTT assay was used to evaluate their anti-hepato-carcinoma bioactivities.Results Two compounds were isolated and elucidated as luteolin and formononetin.Luteolin had strong cytotoxic effect on HepG2 and LO2 with IC50 values of （103.32 ±21.34） and （131.13 ±13.48） μmol/L;Formononetin showed lighter cytotoxic effect on HepG2 with IC50〉200μmol/L.Conclusion Luteolin and formononetin are isolated from Cortex Jug-lans Mandshurica for the first time, and luteolin shows significant anti-tumor activity in human hepatoma cells.