| 食道癌中miR-542-3p对Survivin基因的作用研究 |
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| 引用本文： | 蔡伟明,彭仕骏,吕文强,张志峰.食道癌中miR-542-3p对Survivin基因的作用研究[J].中国医药科学,2011(11):18-19. |
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| 作者姓名： | 蔡伟明 彭仕骏 吕文强 张志峰 |
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| 作者单位： | 广东省揭阳市人民医院心胸外科 |
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| 摘 要： | 目的研究食道癌中Survivin基因是否受某些miRNA的调控,探讨miR-542-3p的表达变化对Survivin基因的影响。方法细胞水平上通过转染miR-542-3p的模拟物,从而过表达miR-542-3p,通过荧光定量PCR检测Survivin基因的表达变化情况。结果 miR-542-3p过表达后,Survivin基因的表达水平显著降低。结论根据上述结果,笔者推测miR-542-3p对Survivin基因具有负调控作用,有利于改善食道癌的恶化,为食道癌的治疗提供一种潜在的手段。
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| 关 键 词： | miR-542-3p Survivin 食道癌 miRNA |
Research in Human Esophageal Squamous Cell Carcinoma by miR-542-3p That Targets Survivin |
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| CAI Weiming,PENG Shijun,LV Wenqiang,ZHANG Zhifeng.Research in Human Esophageal Squamous Cell Carcinoma by miR-542-3p That Targets Survivin[J].China Medicine and Pharmacy,2011(11):18-19. |
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| Authors: | CAI Weiming PENG Shijun LV Wenqiang ZHANG Zhifeng |
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| Institution: | Department of Cardiothoracic Surgery,People’s Hospital of Jieyang,Guangdong Province,Jieyang 522000,China |
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| Abstract: | Objective The inhibitor of apoptosis(IAP)protein survivin was highly expressed in cancers,but not in normal differentiated tissues.In this paper we investigated the influence of miR-542-3p on the expression levels of survivin gene.Methods Human esophageal squamous cell carcinoma cell line(KYSE-510)was transfected with miR-542-3p mimics.Then the survivin expression levels were determined by using real-time PCR.Results The expression levels of survivin gene was significantly down regulated after the transfection of miR-542-3p.Conclusion The results showed that the miR-542-3p may negatively regulated the expression levels of survivin.This suggested that miR-542-3p may possess potential function in the treatment of esophageal carcinoma. |
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| Keywords: | miR-542-3p Surviving Human esophageal squamous cell carcinoma miRNA |
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