预防及超早期应用尼莫同对小鼠局灶性脑梗死的影响

目的观察尼莫同超早期给药对小鼠局灶性脑梗死的影响.方法经右侧颈总动脉将尼龙单丝线栓至大脑中动脉造成永久性缺血模型.经尾静脉及腹腔注射尼莫同,TTC染色、图像分析仪测定脑梗死体积.结果单纯缺血组各时间点脑梗死体积最大,缺血前预防给药组各时间点脑梗死体积最小,缺血后即刻给药组各时间点脑梗死体积介于二者之间.结论小鼠大脑中动脉闭塞后其局灶性脑梗死体积于术后第3天达高峰.缺血前预防给药组、缺血后即刻给药,尼莫同均可使梗死体积减少.

Proventive and ultra-early effects of nimotop on focal cerebral infarct in mice

Objective To investigate the proventive and ultra-early effect of nimotop on focal cerebral infarct in mice.Methods Focal ischemic models of middle cerebral artery occlusion (MCAO) were established by inserting nylon monofilament through the right common carotid artery into the anterior cerebral artery. Nimotop was injected through caudal vein and abdominal cavity. The infarct size was measured by TTC stain and image analyzer.Results The average infarct volume was the largest in the mice without treatment, the smallest in preoperative treatment and the smaller in postoperative treament mice.Conclusions The focal infarct volume reached the top in 3th day after MCAO. Nimotop could reduce the infarct size whether preoperative or postoperative administration.