乙型肝炎病毒母婴垂直传播与人类白细胞抗原-DR区域基因相关性的研究

目的　探讨人类白细胞抗原 (HLA)DR区域基因HLA DR3、HLA DR4、HLA DR13、HLA DR15与乙型肝炎病毒 (HBV)母婴垂直传播的关系。方法　采用聚合酶链反应 序列特异性引物 (PCR SSP)方法 ,检测 78例乙型肝炎表面抗原 (HBsAg)阳性孕妇 (研究组 )和 4 0例正常孕妇 (对照组 )外周静脉血中HLA DR3、HLA DR4、HLA DR13、HLA DR15基因表型分布及频率。结果　 (1)研究组孕妇HLA DR3基因频率为 19 2 % ,显著高于对照组的 5 0 % (P <0 0 5 ) ;HLA DR13基因频率为 2 6 % ,显著低于对照组孕妇的 17 5 % (P <0 0 5 ) ;两组孕妇HLA DR4、HLA DR15的基因频率比较 ,差异无显著性 (P >0 0 5 )。 (2 )研究组孕妇中 ,HBV高复制状态者的HLA DR3基因频率为30 0 % ,显著高于低复制状态者的 7 9% (P <0 0 5 )。 (3)将研究组孕妇分娩的新生儿 ,根据脐血清HBsAg、HBVDNA的检测结果分为 :宫内感染组和宫内未感染组 ,宫内感染组孕妇HLA DR3的基因频率为 5 0 0 % ,显著高于宫内未感染组的 14 7% (P <0 0 5 )。其余各基因表型比较 ,差异无显著性(P >0 0 5 ) ;宫内感染组新生儿HLA DR3基因频率为 30 0 % ,明显高于宫内未感染组的 7 4 % ,但差异无显著性 (P >0 0 5 )。结论　孕妇HBsAg携带与HLA DR3和HLA DR13表达有明显相关

Study on the association between maternal-infantile vertical transmission of hepatitis B virus and human leukocyte antigen DR gene domain

Objective To study on the association between maternal-infantile vertical transmission of hepatitis B virus and human leukocyte antigen(HLA)-DR gene domain. Methods HLA-DR3,HLA-DR4,HLA-DR13,HLA-DR15 were detected by the technique of polymerase chain reaction-sequence specific primers(PCR-SSP) in maternal and neonatal blood of study group(78 cases) and control group(40 cases). Results (1) In the pregnant women of study group, the gene frequency of HLA-DR3 was significantly higher than that in control group(P<0.05),but the frequency of HLA-DR13 was significantly lower than those in control group (P<0.05), while the frequencies of HLA-DR4 and HLA-DR15 had no significant difference between the two groups(P>0.05). (2) The pregnant women with high replication condition of HBV had significantly higher frequency of HLA-DR3 than those with low replication of HBV(P <0.05).There was no significant difference of other phenotype between the two conditions. (3) The frequency of HLA-DR3 of pregnant women in intrauterine infection group was significantly higher than that of intrauterine non-infection group(P<0.05). There had no significant difference of the frequencies of HLA-DR4, HLA-DR13, HLA-DR15 between the two groups(P>0.05). The gene frequencies of HLA-DR3, HLA-DR4, HLA-DR13, HLA-DR15 were found no significant difference between the neonates of the study group and the control group(P>0.05). Although the gene frequency of HLA-DR3(30.0%) in neonates of intrauterine infection group was higher than that of intrauterine non-infection group (7.4%), but no statistical difference was found between the two group(P>0.05). Conclusions HLA-DR3 is positively correlated with chronic HBV carrying and high replication conditions of pregnant women. HLA-DR13 correlated negatively with chronic HBV carrying of the pregnant women.HLA-DR3 carriers of pregnant women and their fetuses have a susceptibility of HBV infection.