| HPLC法测定益肾养元合剂中补骨脂素和异补骨脂素的含量 |
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| 引用本文： | 侯勤,彭惠炫,李春芳. HPLC法测定益肾养元合剂中补骨脂素和异补骨脂素的含量[J]. 今日药学, 2012, 22(4): 219-221 |
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| 作者姓名： | 侯勤 彭惠炫 李春芳 |
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| 作者单位： | 广州潘高寿药业股份有限公司,广东广州,511480 |
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| 摘 要： | 目的 建立HPLC法测定益肾养元合剂中补骨脂素和异补骨脂素的含量.方法 采用AgilentC18柱(1.50 mm x4.6 mm,5μm)为色谱柱;流动相为甲醇∶水(35∶65);检测波长为246nm;流速1.0ml/min;柱温35℃.结果 补骨脂素在40.04 ～800.80 ng范围内有良好的线性关系(r =0.999 95),平均回收率为98.3％,RSD为2.45％;异补骨脂素在40.88 ～817.60 ng范围内有良好的线性关系(r=0.999 96),平均回收率为99.1％,RSD为2.16％;结论 本法简单、准确可靠,可用于该制剂的质量控制.
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| 关 键 词： | 益肾养元合剂 补骨脂素 异补骨脂素 HPLC |
Determination of Psoralen and Isopsoralen in Yishen Yangyuan Heji by HPLC |
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| HOU Qin , PENG Hui-xuan , LI Chun-fang. Determination of Psoralen and Isopsoralen in Yishen Yangyuan Heji by HPLC[J]. Pharmacy Today, 2012, 22(4): 219-221 |
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| Authors: | HOU Qin PENG Hui-xuan LI Chun-fang |
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| Affiliation: | (Guangzhou Pangaoshou Pharmaceutical CO.,LTD,Guangzhou,Guangdong 511480,China) |
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| Abstract: | Objective To esablish a HPLC method for determining the content of psoralen and isopsoralen in Yishen Yangyuan Heji.Methods The Agilent C18 column(150 mm×4.6 mm,5 μm) was used.The mobile phase was consisted by methanol-water(35∶65).The detection wavelength was 246 nm.The flow rate was 1.0 ml/min.column at 35 ℃.Results The lingear range of psoralen was 40.04~800.80 ng(r=0.999 95).The average recovery was 98.3%,RSD was 2.45%.The lingear range of isopsoralen was 40.88~817.60 ng(r=0.999 96).The average recovery was 99.1%,RSD was 2.16%.Conclusion The method is simple,reliable,accurate and can be applied to the quality control of the preparetion. |
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| Keywords: | Yishen Yangyuan Heji psoralen isopsoralen HPLC |
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