抗结核药物凝胶介质肺内应用安全性评价

目的 对卡波姆凝胶和阿胶经支气管镜介入兔肺部后的安全性进行评价,为含抗结核药物凝胶肺内应用提供依据.方法 45只日本长耳兔随机分成对照组、卡波姆组和阿胶组,每组15只,实验周期4周.检测体温、体质量、血常规、T细胞亚群及病理.结果 各组间体温、体质量、血常规及外周血T细胞亚群比较差异均无统计学意义.卡波姆组和阿胶组凝胶介入前与介入后CD3+、CD4+、CD8+百分比比较差异亦无统计学意义.卡波姆组实验动物左肺底细支气管无凝胶成分残留.肺大体及光镜病理观察与对照组相比均未产生明显变化.阿胶组实验动物左肺底细支气管有少量阿胶存留,其中1只实验动物出现花斑肺,光镜病理观察可见肺泡间隔内淋巴细胞浸润.其余14只实验动物肺大体及光镜病理观察与对照组比较均未发现明显变化.结论 卡波姆凝胶和阿胶肺内应用均具有较好的安全性,可作为含抗结核药物凝胶的良好基质.

Evaluation on security of antituberculosis medicament gel administered in lung

Objective To evaluate the effect of carbomer gel and e-gum bronchoscopy intervention in the rabbit lung and provide evidence for using antituberculosis medicament gel administered in lung.Methods Forty-five Japanese long-eared rabbits were randomly divided into control group,carbomer group and e-gum group.Animal temperature,avoirdupois,hemic routine,T lymphocyte subsets,and pathology were tested.Results There were no statistical difference in animal temperature,avoirdupois,hemic routine and T lymphocyte subsets of each group.There were no statistical difference in percent of CD3+,CD4+,CD8+between before and after injection in carbomer group and e-gum group.Lung tissue pathology of carbomer group was no abnormality.There was no carbomer gel in left lung.In e-gum group,a small amount of gel was found in left bronchia of the rabbit.One of rabbits got pneumonia and lymphocyte was found in Spacing of alveolus. Conclusions Carbomer gel and e-gum can be used in lung for security and be good medium to develop antituberculosis medicament gel.