| 牙龈卟啉单胞菌脂多糖对人主动脉内皮细胞表达ICAM-1影响的研究 |
| |
| 引用本文： | 刘斌,程岚,刘大力,汪嘉,张秀丽,束蓉,梁景平. 牙龈卟啉单胞菌脂多糖对人主动脉内皮细胞表达ICAM-1影响的研究[J]. 牙体牙髓牙周病学杂志, 2011, 0(10): 568-572 |
| |
| 作者姓名： | 刘斌 程岚 刘大力 汪嘉 张秀丽 束蓉 梁景平 |
| |
| 作者单位： | 上海交通大学医学院附属第九人民医院·牙体牙髓科;上海交通大学医学院附属第九人民医院·牙周病科;上海市口腔医学研究所上海市口腔医学重点实验室; |
| |
| 基金项目： | 上海市科委基础重点研究项目(09JC1409100) |
| |
| 摘 要： | 目的:分别观察牙龈卟啉单胞菌(P.gingivalis)脂多糖(LPS)和大肠杆菌(E.coli)LPS对人主动脉内皮细胞(HAECs)在转录和翻译水平表达细胞间粘附分子-1(ICAM-1)的影响。方法:在体外分别以P.gingivalisLPS和E.coliLPS刺激HAECs,实时荧光定量PCR技术检测ICAM-1基因表达,蛋白质印迹技术检测ICAM-1膜蛋白表达。采用SPSS18.0软件包中ANOVA对数据进行分析。结果:P.gingivalisLPS作用6 h后开始诱导ICAM-1 mRNA表达,14 h达到高峰,22 h仍有高表达,各时间点与未刺激组相比,差异均有统计学意义(P<0.05);而E.coliLPS作用2 h后即开始诱导ICAM-1 mRNA表达,14 h达到高峰,22 h仍有高表达,各时间点与未刺激组相比差异均有统计学意义(P<0.05)。两种LPS均能在蛋白水平上调ICAM-1的表达,这种上调作用呈浓度和时间依赖,且P.gingivalisLPS的作用较之E.coliLPS要缓和。结论:P.gingivalisLPS能够在转录和翻译水平上调HAECs表达ICAM-1,提示P.gingivalisLPS在动脉粥样硬化(AS)的发生发展过程中可能发挥了重要作用。E.coliLPS可能与急性炎症性疾病有关而与AS无关。
|
| 关 键 词： | 牙龈卟啉单胞菌 脂多糖 人主动脉内皮细胞 细胞间粘附分子-1 |
Porphyromonas gingivalis LPS induces ICAM-1 expression in human aortic endothelial cells |
| |
| LIU Bin,CHENG Lan,LIU Da-li,WANG Jia,ZHANG Xiu-li,SHU Rong,LIANG Jing-ping. Porphyromonas gingivalis LPS induces ICAM-1 expression in human aortic endothelial cells[J]. Chinese Journal of Conservative Dentistry, 2011, 0(10): 568-572 |
| |
| Authors: | LIU Bin CHENG Lan LIU Da-li WANG Jia ZHANG Xiu-li SHU Rong LIANG Jing-ping |
| |
| Affiliation: | LIU Bin,CHENG Lan,LIU Da-li,WANG Jia,ZHANG Xiu-li,SHU Rong,LIANG Jing-ping(*Department of Endodontics and Operative Dentistry,Department of Periodontology,Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai Key Lab of Stomatology,Shanghai 200011,China) |
| |
| Abstract: | AIM: To observe the effects of Porphyromonas gingivalis(P.gingivalis)Lipopolysaccharide(LPS)and E.coli LPS on mRNA and protein expressions of intercellular adhesion molecule-1(ICAM-1)in human aortic endothelial cells(HAECs).METHODS: HAECs were challenged by P.gingivalis LPS or E.coli LPS in vitro.ICAM-1 mRNA was measured by realtime PCR.The protein expression of ICAM-1 was detected by Western blotting assay.The data was statistically analyzed by SPSS 18.0 software package.RESULTS: P.gingivalis LPS as well a... |
| |
| Keywords: | Porphyromonas gingivalis lipopolysaccharide human aortic endothelial cells intercellular adhesion molecule 1 |
| 本文献已被 CNKI 等数据库收录！ |
|
