| 小苦苣菜水提取物中木犀草苷的含量测定 |
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| 引用本文： | 梁惠珍,王丽芳,裴妙荣.小苦苣菜水提取物中木犀草苷的含量测定[J].山西中医,2010,26(5):46-47. |
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| 作者姓名： | 梁惠珍 王丽芳 裴妙荣 |
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| 作者单位： | 1. 山西中医学院,太原,030024
2. 山西中医学院附属第二中医院 |
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| 摘 要： | 目的：采用HPLC法测定小苦苣菜水提取物中木犀草苷的含量。方法：HPLC法,Diamonsil C18柱（4.6 mm×250 mm,5μm）;流动相：乙腈-0.5%冰乙酸水溶液（20∶80）;流速：1.0 mL.min-1;检测波长：350 nm。结果：木犀草苷含量在0.119 2~0.953 6μg范围内线性关系良好,相关系数r=0.9999,平均回收率为98.94%,RSD=1.13%（n=6）。结论：该方法简便,可靠,可用于小苦苣菜药材的质量控制。
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| 关 键 词： | 小苦苣菜 木犀草苷 HPLC |
Determination of Galuteolin in Water - Extract of Ixerlidium Chinense( Thunb.) by HPLC |
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| LIANG Hui-zhen,WANG Li-fang,PEI Miao-rong.Determination of Galuteolin in Water - Extract of Ixerlidium Chinense( Thunb.) by HPLC[J].Shanxi Journal of Traditional Chinese Medicine,2010,26(5):46-47. |
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| Authors: | LIANG Hui-zhen WANG Li-fang PEI Miao-rong |
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| Institution: | (Shanxi TCM College,Taiyuan,030024,China) |
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| Abstract: | Objective： To determine galuteolin in water-extract of Ixerlidium chinense（Thunb.）by HPLC.Method： HPLC was used in the quantitative analysis of galuteolin in the sample.The separation was performed on Diamonsil C18（4.6 mm ×250 mm,5 μm） column with a mixture of acetonitrile-0.5 % acetic acid glacial（20∶80） as mobile phase.The detection wavelength was set at 350 nm and the flow rate was 1.0 mL·min-1.Result： The linear range of galuteolin was 0.119 2 μg-0.953 6 μg（r=0.999 9）,the average recovery was 98.94%,RSD = 1.13%（n=6）.Conclusion： The method is simple,reliable and good in determining galuteolin in Ixerlidium chinense（Thunb.）. |
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| Keywords: | HPLC |
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