解脲支原体的微量菌落形成单位和颜色变化单位的测定

脲支原体的定量计数,因其个体微小,一般需要在光学显微镜下计数平板上生长的菌落,得出菌落形成单位(CFU/ml)和在试管中观察液体培养基pH改变,依颜色变化程度确定颜色变化单位(CCU/ml)。本试验用40孔细胞培养板对解脲支原体进行微量CFU和CCU测定,一块细胞培养板相当于40支小试管和5～6块琼脂平板,但只需要1/150～1/10的常规培养基,经比较微量法与常规法差别不大。这种微量的方法容易做到定量规范化、大大减少了器材和培养基的消耗、缩短了其它的辅助工作量。微量法操作简便、误差小、重复性好,并能在同一块细胞培养板上对多份本进行培养和比较。

COUNTING OF MICRO COLONY FORMATION UNIT AND MICRO COLOR CHANGE UNIT OF UREAPLASMA UREALYTICUM

Usually, tiny colonies of ureaplasma urealyticum on the agar plate are counted under microscope for obtaining colony formation unit/ml ( CFU/ml ) and the pH change of the media is determined with color change unit/ml ( CCU/ml ). In' our laboratory, micro-CFU and micro-CCU methods were devised by using a 40 wells culture plate. One plate needs only 1/150-1/10 of the media used in routine. One plate is equivalent to 40 tubes and 5-6 agar plate. There is no statistical difference in result between micro-and routine method. However, ths micro method is esay to manipulate, consumes less media, reproduces well and can perform several samples with the same plate.