复方健肾颗粒对糖尿病肾病大鼠的初步药效学研究

目的考察复方健肾颗粒对高脂高糖饮食联合小剂量链脲佐菌素(STZ)致糖尿病肾病大鼠的药效学指标的影响,并初步探讨其机制。方法通过高脂高糖饮食联合小剂量STZ进行2型糖尿病肾病动物造模。通过测定空腹血糖(FBG)、血肌酐(Scr)、尿素氮(BUN)、糖化血红蛋白(GHb)、总胆固醇(TC)、三酰甘油(TG)、超氧化物歧化酶(SOD)、丙二醛(MDA)等指标观察其药效,考察其对肾脏病理改变的影响。通过ELISA法测定单核细胞趋化因子-1(MCP-1)、细胞间黏附分子-1(ICAM-1)、白细胞介素-6(IL-6)水平,初步探讨其作用机制。结果与模型组相比,复方健肾颗粒各组大鼠体质量均显著增加(P0.01),大鼠饮食量、饮水量、尿量、尿蛋白定量显著降低(P0.05、0.01),FBG值均下降(P0.05、0.01),Scr均下降(P0.01),BUN均下降(P0.05、0.01),GHb水平显著下降(P0.05、0.01),TC、TG、MDA水平显著下降(P0.01),SOD显著升高(P0.01),IL-6、MCP-1、ICAM-1水平下降显著(P0.01)。结论复方健肾颗粒具有一定的降血糖、调血脂、抗氧化作用,能够保护肾脏的功能,其作用机制可能与MCP-1、ICAM-1、IL-6等炎症通路有关。

Pharmacodynamics research of Compound Jianshen Granules on rats with type 2 diabetic nephropathy

Objective To study the pharmacodynamics effect of Compound Jianshen Granules (CJG) on rats with type 2 diabetic nephopathy induced by high-fat and sugar diet combined low dose streptozotocin (STZ), and to investigate its mechanism. Methods Type 2 diabetic nephropathy animal model was established by feeding with high fat and sugar diet combined with low dose of STZ. FBS, Scr, BUN, GHb, TC, TG, SOD, MDA, etc were measured to study its effects of reducing blood glucose and protecting kidney, data were dealed with SPSS17.0 software and analyzed in statistics, its pharmacodynamic effects were expounded by kidney and pancreas pathological section. MCP-1, ICAM-1, and IL-6 were tested by ELISA to probe into its mechanism tentatively. Results Compared with the model group, the weight of each drug administration group of CJG entirely rised, and the difference had statistical significance (P<0.01); The food, water, urine volume, and urine protein content of each drug administration group of CJG decreased, and the difference had statistical significance (P<0.05, 0.01); The blood glucose level of each drug administration group of CJG decreased, and the difference had statistical significance (P<0.05, 0.01); The Scr and BUN of each drug administration group of CJG decreased, and the difference had statistical significance (P<0.05, 0.01); The GHb of each drug administration group of CJG decreased significantly (P<0.05, 0.01); TC, TG, and MDA of each drug administration group of CJG decreased significantly (P<0.01); SOD of each drug administration group of CJG increased significantly (P<0.01); IL-6, MCP-1, and ICAM-1 of each drug administration group of CJG decreased significantly (P<0.01). Conclusion FBG and each biochemical criterion and pathological section results demonstrate that CJG have the certain effects of decreasing blood sugar and protecting kidney, moreover traeatment groups have significant difference compared with model groups. ELISA determination results indicate that the action mechanism of CJG is potentially related to MCP-1, ICAM-1, and IL-6 inflammatory pathway.