姜黄素对小鼠皮肤移植排斥反应的影响

目的 探讨姜黄素对小鼠皮肤移植排斥反应的免疫抑制作用。方法 BALB/c小鼠作为供体，C57BL/6小鼠作为受体，建立背-背皮肤移植模型。术后分为假手术组、模型组、姜黄素（50 mg/kg）组、环孢素A （10 mg/kg）组、姜黄素+环孢素A （50 mg/kg+5 mg/kg）组，每组10只，每天1次，ip给药10 d。记录各只小鼠移植皮片的存活天数，术后第4、8天采用ELISA法检测血浆白细胞介素-2（IL-2）浓度。结果 姜黄素组、环孢素A组、姜黄素+环孢素A组的小鼠移植皮片存活时间分别为14.77、16.81、19.96 d，与模型组（12.10 d）比较差异显著（P<0.01）；姜黄素+环孢素A组的小鼠移植皮片存活时间与姜黄素或环孢素A组比较显著延长（P<0.05、0.01）；姜黄素组、环孢素A组、姜黄素+环孢素A组术后第4天血浆IL-2水平分别为3.68、2.05、2.70 ng/mL，与模型组（4.76 ng/mL）比较差异显著（P<0.05、0.01）；姜黄素组、环孢素A组、姜黄素+环孢素A组术后第8天血浆IL-2水平分别为4.06、2.11、2.95 ng/mL，与模型组（5.85 ng/mL）比较差异显著（P<0.01）。结论 姜黄素具有抑制皮肤移植小鼠免疫排斥反应的作用。

Immunosuppressive effect of curcumin on skin transplantation in mice

Objective To investigate the immunosuppressive effect of curcumin on xenogenic skin transplantation in mice. Methods The skin transplantation model was established by an operation, tergal skin flaps from BALB/c donor mice transplanted to the back of C57BL/6 recipient mice. Then recipient mice were divided into five groups at random, namely sham, model, curcumin (50 mg/kg), Cyclosporin A (10 mg/kg) and curcumin+Cyclosporin A (50 mg/kg+5 mg/kg). All mice were ip administered once daily for 10 d. The survival days of skin graft were recorded in all groups. The interleukin-2 (IL-2) levels in plasma of all mice were determined by ELISA 4 and 8 d after the operation, respectively. Results The mean survival time of skin graft in curcumin, Cyclosporin A and curcumin+Cyclosporin A groups were 14.77, 16.81 and 19.96 d, respectively, which showed significant differences comparing with 12.10 d of model group (P<0.01). Combination of curcumin and Cyclosporin A administration showed a longer mean survival days than curcumin or Cyclosporin A group (P<0.05 or 0.01). The IL-2 levels in plasma of mice in curcumin, Cyclosporin A and curcumin+Cyclosporin A groups on postoperative day 4 were 3.68, 2.05 and 2.70 ng/mL, respectively, which were significantly reduced than 4.76 ng/mL of model group (P<0.05 or 0.01). The IL-2 levels in plasma of mice in curcumin, Cyclosporin A and combination of curcumin and Cyclosporin A groups on postoperative day 8 were 4.06, 2.11 and 2.95 ng/mL, respectively, which were significantly reduced than 5.85 ng/mL of model group (P<0.01). Conclusion Curcumin may have a good immunosuppressive effect on mice with xenogenic skin transplantation.