一株L-脯氨酸产生菌的选育 |
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引用本文: | 刘党生,贺新芬.一株L-脯氨酸产生菌的选育[J].沈阳药科大学学报,1996,13(2):112-116. |
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作者姓名: | 刘党生 贺新芬 |
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摘 要: | 以天津短杆菌(Brevibacteriumtianjincese)T_(6~13)为出发菌株,采用紫外线连续诱变处理,获得一株抗磺胺胍和利福平的L-异亮氨酸营养缺陷型SF28菌株,该菌株摇瓶发酵产L-脯氨酸28.5g/L,经过培养基成分考查,该菌株在32℃培养72h可产L-脯氨酸37.8g/L。
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关 键 词: | L-脯氨酸 菌种选育 发酵 |
REDING OF AN L-PROLINE PRODUCER |
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Abstract: | An L-isoleucine auxotropy mutant,strain SF28,resistant to sulfaguanidine and rifampin was obtained from Brevibacterium Tianjinese T6~13 by serial treatment with ultraviolet ray.This mutant produces L-proline in shaking flask at 32℃ when fermented for 72 hours,and it can produce 37.8g/L of L-proline on examination of medium composition. |
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Keywords: | L-proline Breeding Fermentation |
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