Detection of Legionella pneumophila by real-time PCR for the mip gene |
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| Authors: | Wilson Deborah A Yen-Lieberman Belinda Reischl Udo Gordon Steve M Procop Gary W |
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| Institution: | Cleveland Clinic Foundation, Cleveland, Ohio, USA. |
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| Abstract: | A real-time PCR assay for the mip gene of Legionella pneumophila was tested with 27 isolates of L. pneumophila, 20 isolates of 14 other Legionella species, and 103 non-Legionella bacteria. Eight culture-positive and 40 culture-negative clinical specimens were tested. This assay was 100% sensitive and 100% specific for L. pneumophila. |
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