| 抗支原体单克隆抗体试剂盒的研制和应用 |
| |
| 引用本文： | 杨保安 孟玲珍. 抗支原体单克隆抗体试剂盒的研制和应用[J]. 军事医学科学院院刊, 1993, 17(1): 36-38 |
| |
| 作者姓名： | 杨保安 孟玲珍 |
| |
| 作者单位： | 军事医学科学院微生物学流行病学研究所(杨保安，孟玲珍，朱曼，厉新奇)，军事医学科学院微生物学流行病学研究所(辛颜彬) |
| |
| 摘 要： | 从20株抗支原体MAb中筛选出1株能够对各种支原体起反应的MAb(3D1)组成试剂盒,用间接免疫荧光法(IFA) 检查了35株传代细胞和6份动物标本,支原体检出率为83%,为了验证此方法的准确性,将其中部分细胞同时做常规培养检查,符合率为90%。结果证明,MAb试剂盒具有特异性高,敏感性强,速度快等优点。
|
| 关 键 词： | 枝原体 单克隆抗体 试剂盒 免疫 |
Development and application of monoclonal antibody diagnostic kit for detecting mycoplasmas |
| |
| Yang Baoan,Meng Lingzhen,Zhu Man,Li Xinqi,Xin Yanbin Institute of Microbiology and Epidemiology,Academy of Military Medical Sciences. Development and application of monoclonal antibody diagnostic kit for detecting mycoplasmas[J]. Bulletin of the Academy of Military Medical Sciences, 1993, 17(1): 36-38 |
| |
| Authors: | Yang Baoan Meng Lingzhen Zhu Man Li Xinqi Xin Yanbin Institute of Microbiology Epidemiology Academy of Military Medical Sciences |
| |
| Abstract: | A hybridoma(3D1) to secrete monoclonal antibody against most mycoplasmascausing cell culture contamination was chosen from 20 hybridomas for assembling a diagnostic kit. Using the diagnostic kit by IFA, 35 samples of continuous cell lines and 6 animal specimens wereexamined. Mycoplasma positive rate was 83%. In order to confirm the specificity and sensitivity ofthe kit to mycoplasmas, monoclonal antibody IFA was compared with routine mycoplasma cul-ture. All positive samples detected by routine mycoplasma culture were positive for monoclonalantibody IFA and a few positive samples detected by monoclonal antibody could not be detectedby routine mycoplasma culture. The results showed that monoclonal antibody IFA was more sen-sitive than routine mycoplasma culture. The monoclonal antibody diagnostic kit provided a rapid,simple and sensitive method for detecting mycoplasmas. |
| |
| Keywords: | mycoplasma monoclonal antibody diagnostic kit immunofluorescence assay |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
