| 抗支原体单克隆抗体试剂盒的研制和应用 |
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| 引用本文： | 杨保安,孟玲珍.抗支原体单克隆抗体试剂盒的研制和应用[J].军事医学科学院院刊,1993,17(1):36-38. |
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| 作者姓名： | 杨保安 孟玲珍 |
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| 作者单位： | 军事医学科学院微生物学流行病学研究所
(杨保安，孟玲珍，朱曼，厉新奇)，军事医学科学院微生物学流行病学研究所(辛颜彬) |
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| 摘 要： | 从20株抗支原体MAb中筛选出1株能够对各种支原体起反应的MAb(3D1)组成试剂盒,用间接免疫荧光法(IFA) 检查了35株传代细胞和6份动物标本,支原体检出率为83%,为了验证此方法的准确性,将其中部分细胞同时做常规培养检查,符合率为90%。结果证明,MAb试剂盒具有特异性高,敏感性强,速度快等优点。
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| 关 键 词： | 枝原体 单克隆抗体 试剂盒 免疫 |
Development and application of monoclonal antibody diagnostic kit for detecting mycoplasmas |
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| Yang Baoan,Meng Lingzhen,Zhu Man,Li Xinqi,Xin Yanbin Institute of Microbiology and Epidemiology,Academy of Military Medical Sciences.Development and application of monoclonal antibody diagnostic kit for detecting mycoplasmas[J].Bulletin of the Academy of Military Medical Sciences,1993,17(1):36-38. |
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| Authors: | Yang Baoan Meng Lingzhen Zhu Man Li Xinqi Xin Yanbin Institute of Microbiology and Epidemiology Academy of Military Medical Sciences |
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| Abstract: | A hybridoma(3D1) to secrete monoclonal antibody against most mycoplasmascausing cell culture contamination was chosen from 20 hybridomas for assembling a diagnostic kit. Using the diagnostic kit by IFA, 35 samples of continuous cell lines and 6 animal specimens wereexamined. Mycoplasma positive rate was 83%. In order to confirm the specificity and sensitivity ofthe kit to mycoplasmas, monoclonal antibody IFA was compared with routine mycoplasma cul-ture. All positive samples detected by routine mycoplasma culture were positive for monoclonalantibody IFA and a few positive samples detected by monoclonal antibody could not be detectedby routine mycoplasma culture. The results showed that monoclonal antibody IFA was more sen-sitive than routine mycoplasma culture. The monoclonal antibody diagnostic kit provided a rapid,simple and sensitive method for detecting mycoplasmas. |
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| Keywords: | mycoplasma monoclonal antibody diagnostic kit immunofluorescence assay |
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