聚羟基丁酸酯作为引导组织再生膜材料的初探

目的:研制一种有一定机械强度又可体内降解的引导组织再生膜,探索聚羟基丁酸酯作为引导组织再生膜的可行性。方法:采用溶剂挥发法制备PHB膜,将膜植入动物体内,分别于1周,1、2、3月取PHB膜及周围组织考察体内降解性,评价组织相容性。结果:溶剂挥发法制备PHB膜具备足够的机械强度,可在体内缓慢降解,具有良好组织相容性。结论:PHB膜能满足作为引导组织再生膜的基本要求,有可能成为一种较理想的引导组织再生的天然膜材料。

A Preliminary Study on Polyhydroxybutyrate Membrane for Guided Tissue Regeneration

Objective:An ideal guided tissue regeneration membrane (GTRm) should possesses good biocompatibility, adequate mechanical strength and biodegradability. Polyhydroxybutyrate(PHB) is a biodegradable hermopolymer that composed by microorganisms. The objective of this study was to explore the possibility of using PHB membrane for guided tissue regeneration.Methods: PHB membrane was prepared by solvent_casting techniques and its physical and chemical properties were examined. Biodegradability and biocompatibility of PHB membranes were inspected by embedding PHB membranes into dogs for 1 week, 1, 2, and 3 months. The scanning electron microscopy (SEM) was used to evaluated biodegradability of PHB membranes, and biocompatibility of PHB membranes was tested by detecting local tissue response with light microscopy (LM).Results: (1)The extension strength and the elongation of fracture of PHB membranes were 28 2 MPa and 1 3% respectively. (2)The morphologic observation of PHB membranes at different terms showed that the surface turned rough because PHB membranes had been biodegraded gradually. (3)The capsule surrounding PHB membrane was mainly consisted of fibrocytes and few lymphocytes. The longer we observed, the thinner the capsule enveloping PHB membrane formed.Conclusion: PHB membranes possess satisfactory mechanical properties, biocompatibility and, can be biodegraded slowly in vivo. PHB membranes could be therefore applied as a biological material for GTR.