1型糖尿病患者血清HCMV DNA与T细胞亚群和胰岛功能的关系研究

目的 研究1型糖尿病患者血清人类巨细胞病毒（HCMV）DNA与外周血T细胞亚群和胰岛功能的相关性。方法 采用定量PCR技术测量20例1型糖尿病患者和40例对照组血清中HCMV DNA含量，并与外周血T细胞亚群、血糖（BG）、胰岛素（Ins)和C肽（C－P）水平进行比较和相关分析。结果 1型糖尿病患者HCMV DNA阳性率及含量明显高于对照组。HCMV DNA阳性患者CD8、空腹及2h BG明显高于对照组，CD4、CD4／CD8比值、空腹及2h Ins和2h C-P明显低于对照组，HCMV DNA含量与CD8、空腹BG呈直线正相关，与CD4／CD8比值、空腹Ins及C－P呈直线负相关。而HCMV DNA阴性患者上述指标与对照组比较差异显著性不及阳性者。结论 1型糖尿病患者HCMV感染与T细胞免疫和胰岛功能异常有关。

Study on correlation between HCMV DNA and T cell subsets as well as islet function in type 1 diabetic patients

Objective To study the correlation between serum human cytomegalovirus (HCMV) DNA and peripheral T cell subsets as well as islet function in type 1 diabetic patients.Methods HCMV DNA levels in sera from 20 type 1 diabetic patients and 40 controls were measured by quantitative polymerase chain reaction (PCR),then the comparison and correlation analysis were made between HCMV DNA and T cell subsets,blood glucose (BG),insulin (Ins) and C peptide (C P).Results The positive rate and levels of HCMV DNA in type 1 diabetic patients were significantly higher than those of controls respectively.The percentage of CD 8 and the levels of fasting BG and BG 2 hours after meal in type 1 diabetic patients with positive HCMV DNA were by far higher than those in controls,while the percentage of CD 4,the ratio of CD 4/CD 8,the levels of fasting Ins,Ins and C P 2 hours after meal were significantly lower than those of controls.There existed the linear positive correlation between HCMV DNA and CD 8 or fasting BG,negative correlation between HCMV DNA and CD 4/CD 8,fasting Ins or fasting C P.Differences were not so significant between type 1 diabetic patients with negative HCMV DNA and controls compared with those between patients with positive HCMV DNA and controls.Conclusion HCMV infection is associated with dysfunction of islet and T cell mediated immunity in type 1 diabetic patients.