卵母细胞体外成熟评价标准的改良研究

目的探讨改良式人未成熟卵母细胞评分标准的可行性和颗粒细胞指标的合理性及其对卵子发育潜能的影响。方法体外培养卵丘-卵母细胞复合体，按改良式人未成熟卵母细胞评分标准，将未成熟卵母细胞分为优质卵和非优质卵，对成熟卵母细胞进行受精，并对胚胎进行评价；用琼脂糖凝胶电泳检测两类卵的颗粒细胞凋亡。结果优质卵的成熟率、卵裂率和优质胚胎率都优于非优质卵，优质卵成熟数和优质胚胎数与非优质卵比较差异有统计学意义（P<0.01）；非优质卵其卵丘细胞DNA凝胶电泳后形成明显DNA 梯带，凋亡程度高于优质卵的卵丘细胞。 结论改良式人未成熟卵母细胞评分标准在临床上具有可行性。

Improvement of the scoring system on human oocytes during maturation in vitro

Objective To study the feasibility of the improved scoring system by evaluating oocytes cultured in vitro,and to discuss the relationship between developmental potential of human immature oocytes and their granulasa cells.Methods Cumulus-enclosed oocyte complexes(COCs) were cultured,evaluated and fertilized.The IVM oocytes were morphologically classified into two groups based on the improved scoring standard.One group was of high quality and the other group was of poor quality.Granulosa cell apoptosis was detected by agarose gel electrophoresis.Results The immature oocyte scoring system was improved.The maturation rate,cleavage rate and high quality embryos of high quality oocytes were better than those of poor quality oocytes.DNA of granulosa cells distilled from poor quality oocytes was discovered as DNA ladder,while that from high quality ones was not.Conclusion The improved scoring standard is feasible in clinical practice.