雷公藤内脂醇对脓毒症大鼠免疫平衡的调节作用

目的：探讨雷公藤内脂醇对脓毒症大鼠血清促炎与抗炎介质的调节作用。方法：90只SD大鼠随机分为3组,每组30只;假手术组（A组），脓毒症模型组（B组）和雷公藤内脂醇治疗组（C组）。A组除不结扎也不刺穿盲肠外，余操作同B组。B组开腹后显露盲肠,根部结扎并刺穿肠壁2次,然后关腹，B组成功后不做任何处理。C组在盲肠结扎和穿孔后立即用雷公藤内脂醇(0.05mg/mL)腹腔注射0.2mg/kg,每隔8ｈ追加1次。各组大鼠在术后6，12，24ｈ分批处死,每批10只。取下腔静脉血检测血清中TNF-α，IL-1，IL-10和TGF-β的水平，并取肝、肾组织行HE及电镜检查。 结果：A组血清中炎症介质水平各时点无明显差异，肝肾功能与形态的变化不明显。B，C组炎症介质水平和抗炎介质水平均显著升高,肝肾功能与形态出现损伤性变化;C组较B组血中两者水平明显降低, 肝肾功能与形态损伤减轻。 结论：雷公藤内脂醇能降低脓毒症大鼠炎症介质水平，即对免疫平衡有调节作用，并减轻肝肾损伤。

Immunoregulation of triptolide on sepsis in rats

Abstract:Objective：To investigate the immunoregulation of triptolide on inflammatory mediators of septic rats. Methods：Ninety SD rats were randomly allocated into three groups: sham operation group (group A), sepsis group (group B)and treatment group with triptolide (group C). There were 30 rats in each group. In group A rats, the abdomen was opened without any other treatment. Sepsis rat model was induced by exposure of caecum and puncturing it,then the abdomen was closed. In group C, triptolide (0.05mg/mL)was given by intraperitoneal injection(0.2mg/kg,Q8h) after ceacum punctured. The rats were killed at 6h,12h and 24h after operation. Then serum levels of TNF-α, IL-6, IL-10, and TGF-β were determined at the three time periods(10 rats for each time period). Tissues from liver and kidney were obtained to observe the changes under microscope and electron microscope. Results：There was no difference between levels of inflammatory mediators at the three time periods in group A, and the functional and morphologic changes of liver and kidney were not significant. Levels of inflammatory mediators were significantly higher in group B and C than those in group A. Pathological changes in liver and kidney were milder in group C than those in group B. Conclusions：Triptolide can lead to down-regulation of levels of inflammatory mediators, exert a modulatory efect on immune balance and alleviate pathological damage of liver and kidney in septic rats.