| 特发性血小板减少性紫癜患者CD3^＋、CD4^＋和CD8^＋T淋巴细胞凋亡率的研究 |
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| 引用本文： | 张静,凌云,曹祥山,邱国强,李敏.特发性血小板减少性紫癜患者CD3^＋、CD4^＋和CD8^＋T淋巴细胞凋亡率的研究[J].临床血液学杂志,2009,22(5):460-462. |
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| 作者姓名： | 张静 凌云 曹祥山 邱国强 李敏 |
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| 作者单位： | 苏州大学附属第三医院常州市第一人民医院血液科;苏州大学附属第三医院常州市第一人民医院肿瘤实验室; |
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| 摘 要： | 目的:通过检测CD3+、CD4+、CD8+T淋巴细胞的凋亡率,探讨T淋巴细胞凋亡在特发性血小板减少性紫癜(ITP)免疫发病机制中的作用。方法:应用流式细胞仪检测ITP患者外周血CD3+、CD4+、CD8+T淋巴细胞的凋亡率;分离ITP患者和正常人外周血单个核细胞(PBMC),分成A、B 2组,A组加入白介素2(IL-2),B组加入IL-2和地塞米松共培养,分别于24和48 h收获细胞,应用流式细胞仪检测CD3+、CD4+、CD8+T淋巴细胞的凋亡率。结果:①ITP患者组CD3+、CD4+、CD8+T淋巴细胞凋亡率均明显低于正常对照(均P<0.01);②细胞培养24 h时ITP患者组A、B 2组间CD3+、CD4+、CD8+T淋巴细胞凋亡率间均差异无统计学意义(均P>0.05),而48 h时B组CD3+、CD4+、CD8+T淋巴细胞的凋亡率均明显高于A组(P<0.05或0.01);正常对照组24和48 h B组CD3+、CD4+、CD8+T淋巴细胞凋亡率均明显高于A组(均P<0.05);③ITP患者组细胞培养24 h后A、B 2组间CD3+、CD4+、CD8+T淋巴细胞凋亡率的差值均明显低于正常对照组(P<0....
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| 关 键 词： | 紫癜 血小板减少性 淋巴细胞 凋亡 |
Study on apoptosis rate of CD3~+,CD4~+ and CD8~+T lymphocytes in patiens with idiopathic thrombocytopenic purpura |
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| ZHANG Jing LING Yun CAO Xiangshan QIU Guoqiang LI Min.Study on apoptosis rate of CD3~+,CD4~+ and CD8~+T lymphocytes in patiens with idiopathic thrombocytopenic purpura[J].Journal of Clinical Hematology,2009,22(5):460-462. |
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| Authors: | ZHANG Jing LING Yun CAO Xiangshan QIU Guoqiang LI Min |
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| Institution: | 1Department of Hematology;Third Affiliated Hosipital of Soochow University;the First People's Hospital of Changzhou;Changzhou;213003;China;2Laboratory of Tumor;Third Affiliated Hospital of Soochow University;the First People's Hospital of Changzhou |
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| Abstract: | Objective:To explore the mechanism of CD3+,CD4+,CD8+T lymphocyte's apoptosis with idiopathic thrombocytopenic purpura.Method:Investigate the apoptotic rate of CD3+,CD4+,CD8+T lymphocytes in ITP patients through flow cytometry.The peripheral blood mononuclear cells seperate from ITP patients was divided into A and B group IL-2 was added in A group IL-2,and dexamethasone was added in B group.After treated with 24 and 48 hours,the PBMCS was havest and the apoptosis rate of CD3+,CD4+,CD8+T lymphocytes was count... |
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| Keywords: | purpura thrombocytopenic lymphocyte apoptosis |
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