3,3''''-二氯联苯胺的急性毒性和体内遗传毒性研究

目的:研究3,3'-二氯联苯胺(DCB)的急性毒性和体内遗传毒性。方法:采用大鼠、小鼠急性毒性试验,小鼠微核试验,小鼠精母细胞染色体畸变试验和小鼠精子畸形试验。结果:经大、小鼠急性毒性试验,得DCB对大鼠、小鼠的LD50分别为4.64、3.16mg/kg,按毒性分级属微毒级物质。在小鼠微核试验中,DCB在高剂量(800mg/kg)时,能诱发小鼠嗜多染红细胞微核率升高(P<0.01);在小鼠精母细胞染色体畸变试验和小鼠精子畸形试验中,DCB在高剂量(216mg/kg)时可分别诱发小鼠精母细胞染色体畸变率和小鼠精子畸形率升高(P<0.01,P<0.01)。结论:在本实验条件下,DCB对实验动物具有一定的遗传毒性作用。

Study on acute toxicity and genotoxicity in vivo of 3,3′-dichlorobenzidine

Objective: To study the acute toxicity and genotoxicity in vivo of 3,3'-dichlorobenzidine. Methods: The oral acute toxicity tests in rats and mice, micronucleus test of polychromatic erythrocytes in bone marrow of mice, chromosomal aberration test of spermatocytes of mice and sperm abnormality test in mice were carried out. Results: LD50 in rats and mice were 4.64 mg/kg and 3.16 mg/kg respectively. In micronucleus test, DCB induced the increase of micronucleus rate in high dose group(800 mg/kg, P<0.01;. In the chromosomal aberration test of spermatocytes and the sperm abnormality test, DCB respectively induced the increase of the chromosomal aberration rate of spermatocytes and the sperm abnormality rate in high dose groups (216 mg/kg, P<0.01, P<0.01;. Conclusion: The genotoxicity of DCB in vivo was found in this study.