肺癌组织中PTEN P63和P27蛋白的表达及意义

目的检测10号染色体同源丢失性磷酸酶-张力蛋白（PTEN）、P63和P27蛋白在肺癌组织中的表达，分析其与肺癌临床病理因素的关系。方法采用免疫组织化学S-P法检测PTEN、P63和P27蛋白在肺癌组织和正常肺组织中的表达情况。结果PTEN、P63和P27在肺癌组织中的阳性表达率分别为27．3％（18／66）、33．3％（22／66）和21．2％（14／66），与三者在正常肺组织中的阳性表达率90．0％、70．0％和70．0％比较差异有统计学意义（P〈0．01）。PTEN和P63在高、中分化鳞癌及低分化鳞癌的阳性表达率分别为55．6％和0．0％（P〈0．01）。PTEN和P63、PTEN和P27、P63和P27的共同阳性表达率分别为13．6％、6．1％和9．1％，PTEN与P63表达呈正相关性（P〈o．01）。Cox多因素分析显示PTEN和P27在模型中呈负相关，患者的预后危险度相对较小，具有统计学意义。结论PTEN、P63和P27蛋白在肺癌组织中较肺正常支气管黏膜上皮表达率低，显示其失表达可能在肺癌的发生中起一定作用。PTEN和P63表达率在高、中分化鳞癌显著高于低分化鳞癌，显示PTEN和P63的表达与肺鳞癌的分化程度有关。PTEN和P63的表达呈正相关性，二者可能有协同作用。PTEN、P63和P27蛋白检测有可能作为诊断肺癌和评价肺癌恶性程度及预后的重要指标。

Expression of PTEN,P63 and P27 protein and its clinical pathological significance in patients with lung cancer

Objective To detect the expression levels of PTEN,P63 and P27 protein in lung cancer tissue,and to elucidate its association with clinical pathological factors for lung cancer. Methods Immunohistochemical S-P ,.nethod was used to examine the expression levels of PTEN,P63 and P27 protein in 66 cases of lung cancer tissue （lung cancer group） and 10 cases of normal lung tissue（control group）. Results The positive expression rates of PTEN,P63 and P27 were significantly higher in lung cancer group than those in control group（27. 3% vs 90.0%; 33.3% vs 70.0% ; 21.2% vs 70.0% ,respectively） ,all P〈9.01. The positive expression rate of PTEN and P63 were both 55.6 % in well and moderately differentiated squamous carcinoma subgroup,and 0.0 % in poorly differentiated squamous carcinoma subgroup. The double positive rate of PTEN and P63, PTEN and P27, as well as P63 and P27 was 13.6% ,6.2% and 9.1% respectively. The expression of PTEN and P63 were positively correlative（P〈0.01）. Multivariate Cox analysis on prognostic factors showed PTEN and P27 were negatively correlative. There was asta tistical significance. Conclusion There is a low expression of PTEN,P63 and P27 protein in lung cancers while a high expression in normal lung tissues,which indicates that the low expression of PTEN,P63 and P27 gene may play a role in the pathogenesis of lung cancer. The positive expression rate of PTEN and P63 is significantly higher in well and moderately differentiated squamous carcinoma tissue than that in poorly differentiated squamous carcinoma tis sue,which indicates that the expression levels of PTEN and P63 are associated with squamous cancer cell differentia- tion degree. The expression of PTEN and P63 are positively correlative,and the two proteins may exert a certain synergistic effect. The determination of PTEN, P63 and P27 protein can be used as an important marker in the diagnosis, evaluation of malignant degree and prognosis of lung cancer.